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Product Spotlight: Coelenterazine
Coelenterazine is a naturally occurring substrate, or luciferin, for a diverse collection of lucifer...
Coelenterazine 400a
Product Description
Coelenterazine 400a is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.
Product Specifications:
Coelenterazine 400a
2,8-Dibenzyl-6-phenyl-imidazo[1,2a]pyrazin-3-(7H)-one
DeepBlueCTM
Formula: C26H21N3O
MW: 391.48 g/mol
Purity: >95% by TLC
Color: Yellow to orange powder
Storage/Handling: Store at -20°C, and protect from light. Keep under inert gas.
Soluble in methanol or ethanol (NOT in DMSO).
Note: It is not recommended to store dissolved coelenterazine 400a at -20°C or -70°C. Coelenterazine 400a is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.
PubChem CID: 135750016
Product Specifications
| Catalog ID | C-320 |
|---|---|
| CAS # | 70217-82-2 |
| MW | 391.48 g/mol |
| Grade | MOLECULAR BIOLOGY GRADE |
| Storage/Handling | Store at -20°C. Protect from light. |
Technical Documentation
P
Protocol
O
Other
C
Certificate of Analysis
S
Safety Data Sheet
Citations
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