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GoldBio’s DH10B Electrocompetent E. coli cells are high efficiency cells, ≥2 x 10⁸ cfu/µg plasmid DNA, suitable for a wide variety of applications requiring high transformation efficiencies, such as cloning and subcloning, as well as working with either cDNA or gDNA library construction. DH10B Electrocompetent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine. Here, we present a detailed protocol for electroporation using DH10B Electrocompetent E. coli cells.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH10B™
Species: E. coli
Transformation efficiency: ≥2 x 10⁸ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s DH10B Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. DH10B Chemically Competent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.

Product Specifications
Competent cell type: Chemically competent
Derivative of: DH10B™
Species: E. coli
Transformation efficiency: ≥8.2 x 10⁶ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s DH5-alpha Electrocompetent E. coli are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. Plasmid quality and yields are both enhanced in our DH5-alpha cells due to mutations in both endA1 and recA1.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 10⁸ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s DH5-alpha Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 10⁶ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s DH10B-Pro™ Electrocompetent E. coli cells are especially designed for the most demanding cloning applications. DH10-Pro™ cells will provide the greatest number of transformants for when your research requires it, including assembling large and multi-DNA fragments, cloning large (≥10 kb up to 350 kb) or difficult construct transformations, working with synthetic bio-applications, and even BAC cloning.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH10B™
Species: E. coli
Transformation efficiency:≥1 x 10⁹ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s BL21 (DE3) Electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature ≥1 x 10⁸ cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1(fhuA2) and are B strain.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 10⁸ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21 (DE3)
Species: E. coli
Transformation efficiency: ≥1 x 10⁶ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s DL39 (DE3) Chemically Competent E. coli cells are engineered with a T7 expression system to transform and express proteins in order to label residues such as phenylalanine or leucine. DL39 (DE3) Chemically Competent E. coli cells are deficient in the aromatic (TyrB), branched-chain (JIvE), and aspartate (AspC) transaminases and are auxotrophic for aspartic acid, isoleucine, leucine, phenylananine, tyrosine, and valine residues.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: DL39
Species: E. coli
Transformation efficiency: ≥1 x 10⁷ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pmp90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted from the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101
Transformation efficiency: ≥2 x 10⁸ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted in the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥5 x 10⁷ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s new ProBlock™ Gold Extra Strength Protease Inhibitor Cocktail is specifically formulated for the most demanding protein purification applications. The optimized concentrations of the various inhibitors in our Extra Strength cocktail deliver an enhanced dose of protease inhibition for the toughest research experiments. ProBlock™ Gold Extra Strength contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases.