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EHA105 Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-225-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-225-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-225-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s EHA105 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Agrobacterium Electrocompetent Cells from GoldBio
GoldBio’s EHA105 Agrobacterium Electrocompetent Cells provide high transformation efficiencies ideal for applications such as cDNA or gDNA library construction.
EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.
Quick Specifications
| Transformation Efficiency | ≥1 x 107 cfu/µg pCAMBIA1391z DNA |
| Reagents for One Reaction | * EHA 105 Electrocompetent Agrobacterium: 25 µl * DNA (pCAMBIA1391z, 500 pg/µl): 1 µl * Recovery medium: 1 ml |
| Plasmid Information | Ti plasmid pEHA105 (pTiBo542DT-DNA) |
| Resistance Information | Rifampicin resistance |
| Plant Information | Rice, tobacco and other plants |
Additional Details
The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif).
Storage/Handling: This product may be shipped on dry ice. EHA105 Agrobacterium Electrocompetent cells should be stored at -80°C, pCAMBIA1391z Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.
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