|CC-225-5x50||5 x 50 μL||$ 199.00|
|CC-225-10x50||10 x 50 μL||$ 359.00|
|CC-225-15x50||15 x 50 μL||$ 485.00|
GoldBio’s EHA105 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GoldBio’s EHA105 Agrobacterium Electrocompetent Cells provide high transformation efficiencies ideal for applications such as cDNA or gDNA library construction.
EHA105 strain also contains an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, which contains the vir gene.
|Transformation Efficiency||≥1 x 107 cfu/µg pCAMBIA1391z DNA|
|Reagents for One Reaction||* EHA 105 Electrocompetent
Agrobacterium: 25 µl
* DNA (pCAMBIA1391z, 500 pg/µl): 1 µl
* Recovery medium: 1 ml
|Plasmid Information||Ti plasmid pEHA105 (pTiBo542DT-DNA)|
|Resistance Information||Rifampicin resistance|
|Plant Information||Rice, tobacco and other plants|
The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif).
Storage/Handling: This product may be shipped on dry ice. EHA105 Agrobacterium Electrocompetent cells should be stored at -80°C, pCAMBIA1391z Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.