Antibody Purification Beads & Resins

Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk from 5-100 ml quantities.

Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).

Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).

Protein A Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein A. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.

Protein A Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)

Binding Capacity: ~10 mg human IgG / ml resin.

Protein G HTC Agarose Beads are high-flow affinity chromatography beads designed for purification and isolation of IgG antibodies from cell culture media, biological fluids, and other antibody-containing samples.

Recombinant Protein G is covalently immobilized on highly cross-linked 4% agarose beads, providing approximately 20 mg human IgG binding capacity per mL of resin. The HTC matrix supports high flow rates and can be used for batch or column purification as well as FPLC, HPLC, and process-scale antibody purification.

Recombinant Protein G contains only the IgG-binding domains of native Protein G. The albumin-binding and cell-associated domains have been removed to maximize specific IgG binding. Protein G binds a broad range of IgG species and subclasses, including antibodies that may have lower affinity for Protein A. Binding capacity can vary depending on the source and subclass of the immunoglobulin, so antibody compatibility should be considered when selecting between Protein G and other affinity ligands.

The highly cross-linked HTC agarose matrix provides the rigidity and mechanical resistance needed for faster flow rates while maintaining good chromatographic performance. Protein G is covalently attached to the matrix to minimize ligand leakage and allow the resin to be regenerated and reused when properly handled. These properties make Protein G HTC Agarose Beads useful for antibody purification, immunoprecipitation, and applications requiring scalable or higher-flow antibody separation.

Binding Capacity

~20 mg Human IgG / ml resin

Protein G Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein G. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.

Protein G Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)

Binding Capacity: ~10 mg human IgG / ml resin.

Protein L Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification and immunoprecipitation. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein L. Magnetic agarose beads provide a convenient and quick method for purifications of IgG, IgM, IgA, and IgD containing kappa light chains from various species without interfering with the antigen binding site.

Protein L Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)

Binding Capacity: ~10 mg human IgG / ml resin.