R_Products

GoldBio’s RR1 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli RR1 is a recA+ derivative of the HB101 strain and can be more useful than HB101 if a recA+ background is required.

Product Specifications
Competent cell type: Chemically competent
Derivative of: RR1
Species: E. coli
Format: Tubes
Transformation efficiency: ≥3 x 10⁷ cfu/µg pUC19 DNA
Blue/white screening: No

SAA1 is a positive acute phase apolipoprotein reactant that associates with HDL. It is expressed predominantly by hepatocytes in the liver and secreted into plasma, but is also expressed and secreted by adipocytes, endothelial cells, and peripheral monocytes. Expression of SAA1 is induced by LPS and by a variety of cytokines such as IL1, IL6, and TNF. Induction causes a dramatic increase of SAA1 levels in blood, indicating that it may play a short-term role in response to injury and inflammation. SAA1 can aggregate into insoluble fibrils that are deposited in various tissues or organs and disrupt normal function, a condition known as amyloidosis. Amyloidosis of SAA1 is associated with rheumatoid arthritis. SAA1 induces the synthesis of a variety of cytokines and proteinases and inhibits proliferation of endothelial cells. When not bound to HDL, it is chemotactic for monocytes, phagocytes, CD4(+) and CD8(+) T-cells, and polymorphonuclear leukocytes. SAA1 is a ligand for CD36/SR-B3, SR-B1, FPRL1, TLR2, and RAGE.

GoldBio growth factors are manufactured for RESEARCH USE ONLY and cannot be sold for human consumption!

Streptavidin Agarose Resin is a high affinity biotin-binding chromatography resin with streptavidin covalently bound to 6% crosslinked agarose beads and a biotin binding capacity of >120 nmol/ml of gel. Immobilized Streptavidin resin is suitable for gravity flow columns, spin columns, and FPLC methods. Immobilized Streptavidin can be used for affinity chromatography purifications by separating biotinylated proteins from nonbiotinylated proteins and for affinity purification of antigens when used with biotinylated antibodies.

Vascular endothelial growth factor is a potent growth factor and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells and promotes angiogenesis and vascular permeability. VEGF is expressed in vascularized tissues and plays a prominent role in normal and pathological angiogenesis. VEGF has been implicated in the induction of tumor metastasis, intra-ocular neovascular syndromes and angiogenesis. Two receptor tyrosine kinases (RTKs), Flt-1 and Flk-1 have been shown to bind VEGF with high affinity.