P_Products

Human parathyroid hormone-like hormone (PTHLH) is a hormone that is expressed in mammary glands, as well as cancer cells. Alternative splicing results in different length isoforms of PTHLH. It is involved in a diverse array of processes. PTHLH plays a key role during endochondral ossification by regulating chondrocyte proliferation, differentiation, and apoptosis. It also regulates epithelial-mesenchymal interactions during development of mammary glands and teeth and interacts with dermal fibroblasts to modulate routine skin cell growth and maintenance. PTHLH is a ligand for the PTH1 receptor that is also affected by PTH. This particular product has been labeled with the stable 15N isotope to help track its activity.

Human parathyroid hormone-like hormone (PTHLH) is expressed in mammary glands as well as cancer cells. Alternative splicing results in different length isoforms of PTHLH. It is involved in a diverse array of processes. PTHLH plays a key role during endochondral ossification by regulating chondrocyte proliferation, differentiation, and apoptosis. It also regulates epithelial-mesenchymal interactions during development of mammary glands and teeth and interacts with dermal fibroblasts to modulate routine skin cell growth and maintenance. PTHLH is a ligand for the PTH1 receptor that is also affected by PTH.

Pleiotrophin (PTN) is a heparin-binding protein, expression of which is developmentally regulated. It is expressed primarily in brain tissues, but is also expressed in lung, kidney, gut, and bone tissues and can be induced by PDGF. PTN is expressed in some tumor cells as well. PTN is involved in a diverse array of functions including mitogenesis, angiogenesis, tissue repair, and neurite outgrowth. It exhibits strong mitogenic activity towards hepatocytes and plays a role in liver regeneration. PTN binds to several different receptors including syndecan-3, PTP-zeta, and ALK. The nucleotide sequence of Human pleiotrophin is about 50% homologous to human midkine (MDK).

Punicalin is an active carbonic anhydrase inhibitor. Additionally, it has been shown to have antioxidative and anti-hepatotoxic effects at small doses. At higher doses, its effect may become hepatotoxic.

Puromycin is an aminonucleoside antibiotic that causes early chain termination within the ribosome during translation. This inhibition of translation is effective in both in vivo and in vitro applications, making it an ideal tool for protein synthesis studies. While selection for E. coli cells is possible with puromycin, it is more commonly and effectively used for mammalian cell selection.

The growth of bacteria, protozoa, algae and mammalian cells is quickly halted by puromycin. However, cells with the pac gene have resistance. Its gene product is puromycin-N-acetyl-transferase which inactivates puromycin through acetylation.

Research has shown puromycin to be effective against certain tumors.

Putrescine attacks s-adenosyl methionine and converts it to spermidine. Spermidine, in turn, attacks another s-adenosyl methionine and converts it to spermine. Putrescine is synthesized in small quantities by healthy living cells by the action of ornithine decarboxylase. The polyamines, of which putrescine is one of the simplest, appear to be growth factors necessary for cell division.

Pyrazinamide is an anti-tubercular antibiotic. It is also used to study ways to prevent liver toxicity and in the formation of polymeric copper complexes.

Although the mechanism of action of pyrazinamide is not well understood, it is thought that upon entering a target cell, it is converted to pyrazinoic acid or POA. This conversion results in altered cellular permeability and decreased internal pH due to POA accumulation in the cytoplasm. This may inhibit fatty acid synthesis.

Simple Stop™ 1 is a broad-spectrum phosphatase inhibitor cocktail consisting of five phosphatase inhibitors in two solutions that target all the phosphatase categories: serine/threonine (Ser/Thr) specific phosphatase, tyrosine specific phosphatase, dual specificity phosphatases, acid phosphatase and alkaline phosphatase.

Each Simple Stop™ phosphatase inhibitor cocktail solution is a 100X concentrated, ready-to-use, solution. Simply add cocktail solution “A” and cocktail solution “B” to your extraction buffers or samples and you are ready to go. This cocktail is ideal for inhibition in tissue extractions and cell lysis experiments and is compatible with most common protein assays.

Simple Stop™ 2 is a broad-spectrum phosphatase inhibitor cocktail consisting of five phosphatase inhibitors in two solutions that target all the phosphatase categories: serine/threonine (Ser/Thr) specific phosphatase, tyrosine specific phosphatase, dual specificity phosphatases, acid phosphatase and alkaline phosphatase.

Each Simple Stop™ phosphatase inhibitor cocktail solution is a 100X concentrated, ready-to-use, solution. Simply add cocktail solution “A” and cocktail solution “B” to your extraction buffers or samples and you are ready to go. This cocktail is ideal for inhibition in tissue extractions and cell lysis experiments and is compatible with most common protein assays.

Simple Stop^TM 3 is a phosphatase inhibitor cocktail consisting of three phosphatase inhibitors that target alkaline and serine/threonine phosphatases. It is a stabilized solution of cantharidin, bromotetramisole and microcystin LR. It is compatible with IEF/2D studies.

The Simple Stop^TM phosphatase inhibitor cocktails are 100X concentrated, ready-to-use solutions that are simply added to your extraction buffers or samples. They are ideal for inhibition in tissue extractions and cell lysis experiments and are compatible with most common protein assays.

Sodium polyanetholesulfonate (SPS) is a homopolymer that was developed to be used as an anticoagulant. It is commonly used in blood culture media for bacteria because it inhibits the antimicrobial activity of immune system components present in blood. SPS inhibits the growth of some microorganisms, but this effect if often media-dependent and can be overcome by addition of gelatin or lysed blood. SPS is a weaker anticoagulant than heparin, most likely due to inhibition of antithrombin III, but a stronger inducer of platelet aggregation. It has also been used to stabilize colloidal suspensions.

GoldBio’s TG1 Phage Display Electrocompetent E. coli cells are suitable for protein expression and preparation of antibody or peptide phage display libraries.TG1 Phage Display cells have ≥1 x 10⁹ cfu/µg efficiency with electroporation and have Amber suppressor strain (supE).

Product Specifications
Competent cell type: ElectroCompetent
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 10⁹ cfu/µg pUC19 DNA
Blue/white screening: Yes