P_Products
Plastic Columns
Catalog ID | Size | Pricing | |
---|---|---|---|
P-301 | 50 x 12 mL | $ 317.00 | |
P-302 | 50 x 35 mL | $ 392.00 |
Description
Gold Biotechnology gravity flow empty columns are ideal for normal protein purification processes. Customers may buy bulk resins and then pack their own His-tag purification columns as an economic option.
These columns serve as tools for gravity purification using small quantities of resin. The package contains 50 plastic columns with their top and end caps.
The plastic columns are polypropylene with a total volume of either 12 mL or 35 mL and contain a polyethylene frit with a nominal pore size of 20 µm. They are specially designed for working with bead volumes of 0.5-2.0 mL (12 mL column) or 2-6 mL (35 mL column).
Plastic Mini Columns
Catalog ID | Size | Pricing | |
---|---|---|---|
P-307-25 | 1 x 25 each | $ 135.00 | |
P-307-100 | 1 x 100 each | $ 492.00 |
Description
Gold Biotechnology gravity flow, Empty Spin Mini Columns are great for small quantity protein purification processes. Customers may buy bulk resins and then pack their own His-tag purification columns as an economic option.
This column serves as a tool for purification using small quantities of resin (100 – 250 µl). It is a single-use format for centrifuge purification.
Product Specifications
Catalog ID | P-307 |
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Plastic Spin Columns
Catalog ID | Size | Pricing | |
---|---|---|---|
P-300 | 800 μL | $ 225.00 |
Description
Gold Biotechnology gravity flow, Empty Spin Columns are an excellent option for small quantity protein purification processes. Customers may buy bulk resins and then pack their own his tag purification columns as an economic option.
The Spin Column serves as a tool for purification using small quantities of resin (50 – 100 µl). It is a single-use format for purifications with syringe (luer lock system) or by centrifugation.
Product Specifications
Catalog ID | P-300 |
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PNPG (Gus)
Catalog ID | Size | Pricing | |
---|---|---|---|
N-325-500 | 500 mg | $ 73.00 | |
N-325-5 | 5 g | $ 400.00 | |
N-325-10 | 10 g | $ 720.00 |
Description
PNPG (Gus) is a chromogenic β-glucuronidase substrate, yielding a yellow solution upon cleavage. It is used to detect E. coli contamination in food and drinking water due to their β-glucuronidase activity.
Product Specifications
Catalog ID | N-325 |
---|---|
CAS # | 10344-94-2 |
MW | 315.24 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store desiccated at -20°C. Protect from light. |
Polyethylene Glycol (PEG) 3350
Catalog ID | Size | Pricing | |
---|---|---|---|
P-290-1 | 1 kg | $ 72.00 |
Description
PEG 3350 is a polyethylene glycol that has an average molecular weight of 3350 g/mol. PEG's are polymers that can be used to precipitate DNA, RNA, proteins and viruses.
Product Specifications
Catalog ID | P-290 |
---|---|
CAS # | 25322-68-3 |
MW | 3350.00 g/mol |
Grade | BIOTECHNOLOGY GRADE |
Storage/Handling | Store desiccated at room temperature. |
Polymyxin B Sulfate
Catalog ID | Size | Pricing | |
---|---|---|---|
P-740-10 | 10 MU | $ 137.00 | |
P-740-100 | 100 MU | $ 527.00 |
Description
Polymyxin B sulfate is a polypeptide antibiotic mixture of B1, B2, and B3 polymyxins. Each of these fractions vary by a fatty acid group and exhibit marginal MIC differences when compared. Polymyxin B is most effective against gram-negative bacteria. It has been shown to work against Pseudomonas aeruginosa, Pseudomonas viridiflava, and Erwinia carotovora. Polymyxin B is freely soluble in aqueous solution.
Product Specifications
Catalog ID | P-740 |
---|---|
CAS # | 1405-20-5 |
MW | 1385.61 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at 4°C. |
Ponceau S Solution 1 mg/mL
Catalog ID | Size | Pricing | |
---|---|---|---|
P-330-SL1 | 1 L | $ 92.00 |
Description
Ponceau S is the sodium salt of a diazo dye used as a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF.It is often used in the staining of polypeptides to be later blot-sequenced because it does not adversely affect the sequencing of the blotted peptides.The stain is also reversible with water washes, preferable for immunological detection, and can even be completely removed.Ponceau S is a negatively charged stain which binds to positively charged amino acids as well as non-polar regions of proteins. The limit of detection for Ponceau S is ~250 ng of protein.
Product Specifications
Catalog ID | P-330-SL |
---|---|
MW | 760.57 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Potassium Iodide, ACS Grade
Catalog ID | Size | Pricing | |
---|---|---|---|
P-440-500 | 500 g | $ 125.00 |
Description
Potassium iodide is an inorganic compound that has many biological and biochemical applications. Potassium iodide is used as a source of iodine in thyrotoxic situations. Potassium iodide is also used to isolate single-stranded DNA (ssDNA), inhibit active oxygen and to quench fluorescent substances. Additionally, it is used as a chaotropic agent a (at high concentrations) to dissolve a gel matrix and can be used in DNA recovery from gel slides by gel dissolution.
Product Specifications
Catalog ID | P-440 |
---|---|
CAS # | 7681-11-0 |
MW | 166.00 g/mol |
Storage/Handling | Store desiccated at room temperature. |
PPBP (CXCL7), Human
Catalog ID | Size | Pricing | |
---|---|---|---|
2110-07-10 | 10 μg | $ 165.00 |
Description
PPBP is an ELR type of CXC chemokine. This recombinant protein is a mature, proteolytically processed form of PPBP containing 70 amino acids that is also known as NAP2. It is expressed in human monocytes after treatment with bacterial endotoxins and released from the alpha-granules of platelets after their activation. NAP2 is chemotactic for fibroblasts and neutrophils. It is involved in a variety of cellular processes including mitogenesis, DNA synthesis, extracellular matrix synthesis, glycolysis, and plasminogen activator synthesis.
Product Specifications
Catalog ID | 2110-07 |
---|---|
MW | ~7.6 kDa |
Storage/Handling | Store desiccated at -20°C. |
PPBP (CXCL7), Rat
Catalog ID | Size | Pricing | |
---|---|---|---|
2510-07-10 | 10 μg | $ 165.00 |
Description
Rat PPBP is an ELR type of CXC chemokine. This recombinant protein is a mature, proteolytically processed form of PPBP that is also known as NAP2. It is expressed in monocytes and macrophages, especially from the lungs, after treatment with bacterial endotoxins and released from the alpha-granules of platelets after their activation. NAP2 is chemotactic for fibroblasts and neutrophils and binds to the receptor CXCR2. It is involved in a variety of cellular processes including mitogenesis, DNA synthesis, extracellular matrix synthesis, glycolysis, and plasminogen activator synthesis.
Product Specifications
Catalog ID | 2510-07 |
---|---|
MW | ~6.8 kDa |
Storage/Handling | Store desiccated at -20°C. |
Praziquantel
Catalog ID | Size | Pricing | |
---|---|---|---|
P-760-25 | 25 g | $ 188.00 |
Description
Praziquantel is an anthelmintic drug used against schistosomes and cestodes. It functions by increasing the tegmentum permeability to calcium ions. This induces paralytic muscle contraction and death. Praziquantel is more effective on adult parasites than young parasites. It suppresses glucose uptake and stimulates lactate release.
Praziquantel is used to study voltage-gated calcium channels.
Product Specifications
Catalog ID | P-760 |
---|---|
CAS # | 55268-74-1 |
MW | 312.41 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
PRL, Murine
Catalog ID | Size | Pricing | |
---|---|---|---|
3310-10-10 | 10 μg | $ 115.00 |
Description
Murine Prolactin (PRL) is a peptide hormone that is secreted most notably from the pituitary gland where it acts in an endocrine fashion, but also from extra pituitary regions where it acts in an autocrine or paracrine fashion. PRL serves as a ligand for the prolactin receptor, PRLR. It is listed as having over 300 separate functions and is involved in more processes than any other pituitary hormone. PRL is most commonly known for its lactation stimulating effect, but also plays a role in reproductive behavior, homeostasis of electrolytes, growth and development, regulation of metabolism, immune system regulation, angiogenesis, and survival of cancer cells. Homozygous female knock-out mice lacking expression of PRL become infertile, whereas homozygous males were fully fertile.
Product Specifications
Catalog ID | 3310-10 |
---|---|
MW | ~22.4 kDa |
Storage/Handling | Store desiccated at -20°C. |
PRL, Rat
Catalog ID | Size | Pricing | |
---|---|---|---|
3510-10-10 | 10 μg | $ 115.00 |
Description
Rat Prolactin (PRL) is a peptide hormone that is secreted most notably from the pituitary gland where it acts in an endocrine fashion, but also from extra pituitary regions where it acts in an autocrine or paracrine fashion. It is listed as having over 300 separate functions and is involved in more processes than any other pituitary hormone. PRL is most commonly known for its lactation stimulating effect, but also plays a role in reproductive behavior, homeostasis of electrolytes, growth and development, regulation of metabolism, immune system regulation, angiogenesis, and survival of cancer cells. PRL acts as a ligand for the prolactin receptor, PRLR.
Product Specifications
Catalog ID | 3510-10 |
---|---|
MW | ~22.6 kDa |
Storage/Handling | Store desiccated at -20°C. |
ProBlock™ Gold 2D Protease Inhibitor Cocktail (EDTA Free) [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-109-1 | 1 mL | $ 205.00 |
Description
ProBlock™ Gold 2D is a protease inhibitor cocktail specifically developed for sample preparation for IEF/2D- studies. No EDTA is used, which allows optimal action of nuclease activity for removing nucleic acids from the samples.
ProBlock™ Gold 2D contains both irreversible and reversible protease inhibitors and inhibits serine, cysteine and metalloproteases etc. Due to the optimized concentration of the various inhibitors, the ProBlock™ Gold 2D shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins extracted from animal cells / tissues, plants, yeast and bacteria etc.
ProBlock™ Gold 2D at 1X concentration in extraction buffer at pH 7-8 inhibits over 90% of protease activities.
Product Specifications
Catalog ID | GB-109 |
---|---|
Storage/Handling | Store at -20°C. |
ProBlock™ Gold Bacterial 2D Protease Inhibitor Cocktail [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-376-1 | 1 mL | $ 79.00 | |
GB-376-5 | 5 mL | $ 299.00 |
Description
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail. ProBlock™ Gold Bacterial 2D contains optimized concentrations of various bacterial protease inhibitors, which provide excellent inhibition of protease activities during recombinant protein purification from bacteria. The cocktail contains both reversible and irreversible protease inhibitors to inhibit metallo, serine and cysteine proteases. It also contains specific inhibitors for bacterial proteases, such as aspartic proteases and aminopeptidases. A 1X concentration of Bacterial ProteaseArrest™ inhibits over 90% of protease activities.
ProBlock™ Gold Bacterial 2D cocktail does not use EDTA as its metalloprotease inhibitor as it would inhibit the activity of proteins that require divalent cations (Ca2+, Mg2+, or Mn2+) for their biological activity. In addition, EDTA would inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC), for example His tagged or CBP tagged proteins. ProBlock™ Gold Bacterial 2Dcocktail is compatible with immobilized metal affinity chromatography.
Product Specifications
Catalog ID | GB-376 |
---|---|
Storage/Handling | Store at 4°C. |
ProBlock™ Gold Bacterial Protease Inhibitor Cocktail [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-330-1 | 1 mL | $ 85.00 | |
GB-330-5 | 5 mL | $ 295.00 |
Description
ProBlock™ Gold Bacterial Protease Inhibitor Cocktail contains optimized concentrations of various bacterial protease inhibitors, which provide excellent inhibition of protease activities during protein purification from bacteria. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine and cysteine proteases. It also contains specific inhibitors for bacterial proteases, such as aspartic proteases and aminopeptidases. A 1X concentration of ProBlock™ Gold Bacterial inhibits over 90% of protease activities.
Some proteins require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.
ProBlock™ Gold Bacterial is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.
Product Specifications
Catalog ID | GB-330 |
---|---|
Storage/Handling | Store at 4°C. |
ProBlock™ Gold Extra Strength Protease Inhibitor Cocktail
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-116-2 | 2 mL | $ 175.00 | |
GB-116-5 | 5 mL | $ 345.00 | |
GB-116-10 | 10 mL | $ 605.00 |
Description
GoldBio’s new ProBlock™ Gold Extra Strength Protease Inhibitor Cocktail is specifically formulated for the most demanding protein purification applications. The optimized concentrations of the various inhibitors in our Extra Strength cocktail deliver an enhanced dose of protease inhibition for the toughest research experiments. ProBlock™ Gold Extra Strength contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases.
Product Specifications
Catalog ID | GB-116 |
---|---|
Storage/Handling | Store at 4°C. |
ProBlock™ Gold Mammalian Protease Inhibitor Cocktail [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-331-1 | 1 mL | $ 85.00 | |
GB-331-5 | 5 mL | $ 295.00 |
Description
ProBlock™ Gold mammalian protease inhibitor cocktail contains optimized concentrations of various mammalian protease inhibitors, which provide excellent inhibition of protease activities during protein purification from mammalian cells and tissues. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine and specific inhibitors for mammalian proteases, such as aminopeptidases. A 1X concentration of ProBlock™ Gold Mammalian inhibits over 90% of protease activities.
Some proteins require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.
ProBlock™ Gold Mammalian is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.
Product Specifications
Catalog ID | GB-331 |
---|---|
Storage/Handling | Store at 4°C. |
ProBlock™ Gold Plant Protease Inhibitor Cocktail [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-332-1 | 1 mL | $ 79.00 | |
GB-332-5 | 5 mL | $ 289.00 |
Description
ProBlock™ Gold Plant protease inhibitor cocktail contains optimized concentrations of various plant protease inhibitors, which provide excellent inhibition of protease activities during protein purification from plant. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine, metalloproteases and specific inhibitors for plant proteases, such as aspartic proteases and aminopeptidases. A 1X concentration oft ProBlock™ Gold Plant inhibits over 90% of protease activities.
Product Specifications
Catalog ID | GB-332 |
---|---|
Storage/Handling | Store at 4°C. |
ProBlock™ Gold Protease Inhibitor Cocktail [100X]
Catalog ID | Size | Pricing | |
---|---|---|---|
GB-108-2 | 2 mL | $ 125.00 | |
GB-108-5 | 5 mL | $ 255.00 | |
GB-108-10 | 10 mL | $ 449.00 |
Description
Due to the optimized concentration of the various inhibitors, the ProBlock™ Gold shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins during purification from animal tissues, plant tissues, yeast and bacteria. ProBlock™ Gold contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. An separate bottle of EDTA is provided to inhibit metalloproteases.
Since some proteins require divalent cations like Ca2+, Mg2+ or Mn2+ for their biological activity, the presence of EDTA may be detrimental to the protein activity. Furthermore, if the protein of interest is purified using immobilized metal chelate affinity chromatography, EDTA must be removed from the buffer before the chromatography. The ProBlock™ Gold is therefore supplied with a separate, optional EDTA solution which may be added to the extraction buffer or lysate as needed.
Product Specifications
Catalog ID | GB-108 |
---|---|
Storage/Handling | Store at 4°C. |