Loading Dye

Gold Biotechnology has affordable chemical dyes for use in loading dyes in gel electrophoresis. GoldBio is committed to providing superior products with superior pricing. We offer Bromophenol Blue, Orange G and Xylene Cyanol FF which migrate similar to DNA bands of 500bp, 50bp and 4kb respectively in a 1% agarose gel. These dyes may be mixed to ficoll or glycerol to create a precise, customized loading dye specific to your research needs.
Showing 1-7 of 7 items.

6X Blue Loading Dye

Catalog ID Size Pricing
L002-5 5 mL $ 29.00
L002-10 2 x 5 mL $ 59.00
L002-20 4 x 5 mL $ 109.00
L002-50 10 x 5 mL $ 249.00

Description

GoldBio's 6x Blue DNA Loading Dye is pre-mixed buffer designed for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 4,000 bp and 500 bp on a standard 1% TAE agarose gel respectively (800 bp and 150 bp on a 2% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Product Specifications

Catalog ID L002
Storage/Handling Store at 4°C.
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Packs & Pricing
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L002-5 2-3 Weeks 5 mL $ 29.00
L002-10 2-3 weeks 2 x 5 mL $ 59.00
L002-20 2-3 weeks 4 x 5 mL $ 109.00
L002-50 2-3 weeks 10 x 5 mL $ 249.00

6X GelRed™ Prestain Loading Buffer with Orange Tracking Dye

Catalog ID Size Pricing
G-735-1 1 mL $ 94.00

Description

GelRed™ is an ultra-sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed™ Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer and eliminates the need to add fluorescent DNA dye to the agarose gel during casting.

6X GelRed™ Prestain Loading Buffer with Orange Tracking Dye contains an orange electrophoresis tracking dye that run at approximately 50 bp in a 1% agarose gel.

GelRed™ and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed without changing your existing imaging system. In addition, GelRed™ is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed™ is compatible with downstream applications such as sequencing and cloning. GelRed™ is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.

Product Specifications

Catalog ID G-735
Storage/Handling Store at 4°C. Protect from light.
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Packs & Pricing
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G-735-1 In stock 1 mL $ 94.00

6X Green Loading Dye

Catalog ID Size Pricing
L001-5 5 ml $ 29.00
L001-10 2 x 5 ml $ 59.00
L001-20 4 x 5 ml $ 109.00
L001-50 10 x 5 ml $ 249.00

Description

GoldBio's 6x Green DNA Loading Dye is a pre-mixed buffer for tracking DNA samples during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 4,000 bp and 50 bp on a standard 1% TAE agarose gel respectively (800 bp and 30 bp on a 2% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Product Specifications

Catalog ID L001
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
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L001-5 In stock 5 ml $ 29.00
L001-10 In stock 2 x 5 ml $ 59.00
L001-20 In stock 4 x 5 ml $ 109.00
L001-50 In stock 10 x 5 ml $ 249.00

Bromocresol Green, Sodium Salt, ACS Grade

Catalog ID Size Pricing
B-091-10 10 g $ 72.00
B-091-25 25 g $ 149.00
B-091-50 50 g $ 238.00
B-091-100 100 g $ 357.00

Description

Bromocresol Green sodium salt is a tracking dye for RNA gels with a pH range of 3.8 (yellow) to 5.4 (blue).

Product Specifications

Catalog ID B-091
CAS # 62625-32-5
MW 720.00 g/mol
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Packs & Pricing
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B-091-10 2-3 Weeks 10 g $ 72.00
B-091-25 2-3 Weeks 25 g $ 149.00
B-091-50 2-3 Weeks 50 g $ 238.00
B-091-100 2-3 Weeks 100 g $ 357.00

Bromophenol Blue Free Acid, ACS Grade

Catalog ID Size Pricing
B-092-5 5 g $ 39.00
B-092-25 25 g $ 49.00
B-092-100 100 g $ 115.00

Description

Bromophenol blue is a dye for tracking proteins and nucleic acids in gel electrophoresis. Bromophenol blue migrates at about the same rate as ~500bp DNA in a 1% agarose gel and at the buffer front in protein polyacrylamide gels.

Product Specifications

Catalog ID B-092
CAS # 115-39-9
MW 669.96 g/mol
Storage/Handling Store desiccated at room temperature.
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Packs & Pricing
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B-092-5 In stock 5 g $ 39.00
B-092-25 In stock 25 g $ 49.00
B-092-100 In stock 100 g $ 115.00

Orange Loading Dye

Catalog ID Size Pricing
L003-5 5 mL $ 29.00
L003-10 2 x 5 mL $ 59.00
L003-20 4 x 5 mL $ 109.00
L003-50 10 x 5 mL $ 249.00

Description

GoldBIo Orange 6x DNA Loading Dye is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains one dye (Orange G) for tracking the DNA migration.

The Orange G migrates at approximately 50 bp on a standard 1% TAE agarose gel (30 bp on 2% TAE agarose gel). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Composition

  • 0.15% Orange G
  • 10mM Tris-HCl (pH 8.0)
  • 60% glycerol
  • 60mM EDTA

Product Specifications

Name(s) Orange Loading Dye
Storage/Handling Store at 4°C for up to 12 months.
Store at -20°C for up to 36 months.
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Packs & Pricing
,
L003-5 2-3 Weeks 5 mL $ 29.00
L003-10 2-3 weeks 2 x 5 mL $ 59.00
L003-20 2-3 weeks 4 x 5 mL $ 109.00
L003-50 2-3 weeks 10 x 5 mL $ 249.00

Tricolor Loading Dye

Catalog ID Size Pricing
L004-5 5 mL $ 29.00
L004-10 2 x 5 mL $ 59.00
L004-20 4 x 5 mL $ 109.00
L004-50 10 x 5 mL $ 249.00

Description

GoldBio Tri-Color 6x DNA Loading Dye is a pre-mixed buffer for tracking DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue and Orange G) for tracking the DNA migration.

The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively (800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively). Glycerol is used to keep the DNA at the bottom of the well. The presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Composition

  • 0.03% Xylene cyanol FF
  • 0.03% Bromophenol blue
  • 0.15% Orange G
  • 10mM Tris-HCl (pH 8.0)
  • 60% glycerol
  • 60mM EDTA

Product Specifications

Name(s) Tri-Color Loading Dye
Storage/Handling Store at 4°C for up to 12 months.
Store at -20°C for up to 36 months.
View
Packs & Pricing
,
L004-5 2-3 Weeks 5 mL $ 29.00
L004-10 2-3 weeks 2 x 5 mL $ 59.00
L004-20 2-3 weeks 4 x 5 mL $ 109.00
L004-50 2-3 weeks 10 x 5 mL $ 249.00

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