Interleukins

Gold Biotechnology has a wide range of Interleukin growth factors from multiple species, including human, murine, rat, canine and rhesus monkey. Interleukins are a large family of cytokines which are soluble hormone-like mediators of the immune system. They were first seen to be expressed by white blood cells (leukocytes), but have subsequently been found to be produced by a wide variety of body cells. Interleukins are involved in processes of cell activation, cell differentiation, proliferation, and cell-to-cell interactions. The expression of interleukins is usually strictly regulated, i.e., the factors are often not secreted constitutively. Interleukins are most often synthesized after cell activation as a consequence of a physiological or non-physiological stimulus.
Showing 61-80 of 93 items.

IL36A (IL-36 alpha), Human (153 a.a.)

Catalog ID Size Pricing
1110-36B-10 10 μg $ 165.00
1110-36B-100 100 μg $ 1,315.00
1110-36B-500 500 μg $ 2,535.00

Description

Interleukin-36A (IL36A) is a pro-inflammatory cytokine that plays an important role in the pathophysiology of several diseases. IL36A, IL36B and IL36G (formerly IL1F6, IL1F8, and IL1F9) are IL1 family members that signal through the IL1 receptor family members IL1Rrp2 (IL1RL2) and IL1RAcP. Studies have shown that IL36A is mainly found in the skin and lymphoid tissues, as well as fetal brain, trachea, stomach and intestine. Notably, IL36A is the only novel IL1 family member expressed on T-cells. IL36A contains 153 amino acid residues, which make up a single nonglycosylated polypeptide that shares 57-68% amino acid sequence identity with mouse, rabbit, equine and bovine IL36A, and 27-57% amino acid sequence identity with other novel IL1 family members.

Product Specifications

Catalog ID 1110-36B
MW ~17.1 kDa
Storage/Handling Store desiccated at -20°C.
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1110-36B-10 3-4 Weeks 10 μg $ 165.00
1110-36B-100 3-4 Weeks 100 μg $ 1,315.00
1110-36B-500 3-4 Weeks 500 μg $ 2,535.00

IL36A (IL-36 alpha), Human (158 a.a.)

Catalog ID Size Pricing
1110-36A-10 10 μg $ 165.00

Description

Interleukin-36A (IL36A) is a pro-inflammatory cytokine that plays an important role in the pathophysiology of several diseases. IL36A, IL36B and IL36G (formerly IL1F6, IL1F8, and IL1F9) are IL1 family members that signal through the IL1 receptor family members IL1Rrp2 (IL1RL2) and IL1RAcP. Studies have shown that IL36A is mainly found in the skin and lymphoid tissues, as well as fetal brain, trachea, stomach and intestine. Notably, IL36A is the only novel IL1 family member expressed on T-cells. IL36A contains 158 amino acid residues which make up a single nonglycosylated polypeptide that is 30% homologous in amino acid sequence to IL1ra, and 27%, 31%, 36%, 46%, 57% and 28% homologous to IL1G, IL36Ra/IL1F5, IL37/IL1F7, IL36G/IL1F8, IL36H/IL1F9 and IL1F10 respectively.

Product Specifications

Catalog ID 1110-36A
MW ~17.7 kDa
Storage/Handling Store desiccated at -20°C.
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1110-36A-10 3-4 Weeks 10 μg $ 165.00

IL36A (IL-36 alpha), Murine (153 a.a.)

Catalog ID Size Pricing
1310-36B-10 10 μg $ 165.00

Description

Interleukin-36A (IL36A) is a pro-inflammatory cytokine that plays an important role in the pathophysiology of several diseases. IL36A, IL36B, and IL36G (formerly IL1F6, IL1F8, and IL1F9) are all IL1 family members that signal through the IL1 receptor family members IL1Rrp2 (IL1RL2) and IL1RAcP. IL36A is mainly found in skin and lymphoid tissues, but can also be found in fetal brain, trachea, stomach and intestine. IL36A is expressed by monocytes, B-cells and T-cells. Notably, IL36A is the only novel IL1 family member expressed on T-cells. Recombinant murine IL36A contains 153 amino acid residues, which compose a single nonglycosylated polypeptide. Specifically, mouse IL36A shares 83% amino acid sequence identity with rat IL36A, 54-60% with human, rabbit, equine and bovine IL36A.

Product Specifications

Catalog ID 1310-36B
MW ~17.1 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
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1310-36B-10 3-4 Weeks 10 μg $ 165.00

IL36A (IL-36 alpha), Murine (160 a.a.)

Catalog ID Size Pricing
1310-36A-10 10 μg $ 165.00
1310-36A-100 100 μg $ 1,315.00
1310-36A-500 500 μg $ 2,535.00

Description

Interleukin-36A (IL36A) is a pro-inflammatory cytokine, which plays an important role in the pathophysiology of several diseases. IL36A, IL36B, and IL36G (formerly IL1F6, IL1F8, and IL1F9) are all IL1 family members that signal through the IL1 receptor family members IL1Rrp2 (IL1RL2) and IL1RAcP. IL36A is mainly found in skin and lymphoid tissues, but can also be found in the fetal brain, trachea, stomach and intestine. IL36A is expressed by monocytes, B-cells and T-cells. Notably, IL36A is the only novel IL1 family member expressed on T-cells. Recombinant murine IL36A contains 160 amino acid residues which compose a single nonglycosylated polypeptide. Specifically, mouse IL36A shares 83% amino acid sequence identity with rat IL36A, 54-60% with human, rabbit, equine and bovine IL36A.

Product Specifications

Catalog ID 1310-36A
MW ~18.0 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1310-36A-10 3-4 Weeks 10 μg $ 165.00
1310-36A-100 3-4 Weeks 100 μg $ 1,315.00
1310-36A-500 3-4 Weeks 500 μg $ 2,535.00

IL36B (IL-36 beta), Human (153 a.a.)

Catalog ID Size Pricing
1110-36D-10 10 μg $ 165.00
1110-36D-100 100 μg $ 1,315.00
1110-36D-500 500 μg $ 2,535.00

Description

Interleukin-36B (IL36B) is a pro-inflammatory cytokine that plays an important role in the pathophysiology of several diseases. IL36A, IL36B and IL36G (formerly IL1F6, IL1F8, and IL1F9) are IL1 family members that signal through the IL1 receptor family members IL1Rrp2 (IL1RL2) and IL1RAcP. IL36B has been reported to be expressed at higher levels in psoriatic plaques rather than symptomless psoriatic skin or healthy control skin. Furthermore, IL36B can stimulate production of IL6 and IL8 in synovial fibroblasts, articular chondrocytes and mature adipocytes.

Product Specifications

Catalog ID 1110-36D
MW ~17.2 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1110-36D-10 3-4 Weeks 10 μg $ 165.00
1110-36D-100 3-4 Weeks 100 μg $ 1,315.00
1110-36D-500 3-4 Weeks 500 μg $ 2,535.00

IL36B (IL-36 beta), Human (157 a.a.)

Catalog ID Size Pricing
1110-36C-10 10 μg $ 165.00
1110-36C-100 100 μg $ 1,315.00
1110-36C-500 500 μg $ 2,535.00

Description

Interleukin-36B (IL36B) is a pro-inflammatory cytokine that plays an important role in the pathophysiology of several diseases. IL36A, IL36B and IL36G (formerly IL1F6, IL1F8, and IL1F9) are IL1 family members that signal through the IL1 receptor family members IL1Rrp2 (IL1RL2) and IL1RAcP. IL36B has been reported to be expressed at higher levels in psoriatic plaques rather than symptomless psoriatic skin or healthy control skin. Furthermore, IL36B can stimulate production of IL6 and IL8 in synovial fibroblasts, articular chondrocytes and mature adipocytes. Human IL36B shares low sequence identity with IL1B, IL36RN, IL36A and IL36G.

Product Specifications

Catalog ID 1110-36C
MW ~17.7 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
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1110-36C-10 3-4 Weeks 10 μg $ 165.00
1110-36C-100 3-4 Weeks 100 μg $ 1,315.00
1110-36C-500 3-4 Weeks 500 μg $ 2,535.00

IL36B (IL-36 beta), Murine (153 a.a.)

Catalog ID Size Pricing
1310-36D-10 10 μg $ 165.00
1310-36D-100 100 μg $ 1,315.00
1310-36D-500 500 μg $ 2,535.00

Description

Interleukin 36B (IL36B) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL36A, IL36B, and IL36G (formerly IL1F6, IL1F8, and IL1F9) are all IL1 family members that signal through the IL1 receptor family members IL1Rrp2 (IL1RL2) and IL1RAcP. IL36B is reported to be expressed at higher levels in psoriatic plaques than in symptomless psoriatic skin or healthy control skin. IL36B can also stimulate the production of IL6 and IL8 in synovial fibroblasts, articular chondrocytes and mature adipocytes.

Product Specifications

Catalog ID 1310-36D
MW ~17.4 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1310-36D-10 3-4 Weeks 10 μg $ 165.00
1310-36D-100 3-4 Weeks 100 μg $ 1,315.00
1310-36D-500 3-4 Weeks 500 μg $ 2,535.00

IL36B (IL-36 beta), Murine (183 a.a.)

Catalog ID Size Pricing
1310-36C-10 10 μg $ 165.00
1310-36C-100 100 μg $ 1,315.00
1310-36C-500 500 μg $ 2,535.00

Description

Interleukin-36B (IL36B) is a pro-inflammatory cytokine which plays an important role in the pathophysiology of several diseases. IL36A, IL36B, and IL36G (formerly IL1F6, IL1F8, and IL1F9) are all IL1 family members that signal through the IL1 receptor family members IL1Rrp2 (IL1RL2) and IL1RAcP. IL36B is reported to be expressed at higher levels in psoriatic plaques than in symptomless psoriatic skin or healthy control skin. IL36B can also stimulate the production of IL6 and IL8 in synovial fibrobasts, articular chondrocytes and mature adipocytes.Within the IL1 family, IL36B/IL1F8 shares 30%, 32%, 37%, 46%, 34%, 45% and 28% amino acid sequence identity with IL1RN, IL1B, IL36RN/IL1F5, IL36A/IL1F6, IL37/IL1F7, IL36G/IL1F9 and IL1F10, respectively.

Product Specifications

Catalog ID 1310-36C
MW ~20.9 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1310-36C-10 3-4 Weeks 10 μg $ 165.00
1310-36C-100 3-4 Weeks 100 μg $ 1,315.00
1310-36C-500 3-4 Weeks 500 μg $ 2,535.00

IL36G (IL-36 gamma), Human (152 a.a.)

Catalog ID Size Pricing
1110-36F-10 10 μg $ 165.00
1110-36F-100 100 μg $ 1,315.00
1110-36F-500 500 μg $ 2,535.00

Description

Interleukin-36G (IL36G) is a pro-inflammatory cytokine that plays an important role in the pathophysiology of several diseases. IL36A, IL36B and IL36G (formerly IL1F6, IL1F8, and IL1F9) are IL1 family members that signal through the IL1 receptor family members IL1Rrp2 (IL1RL2) and IL1RAcP. IL36G is secreted when transfected into 293-T cells and could constitute part of an independent signaling system analogous to that of IL1A and IL1B receptor agonist and interleukin-1 receptor type I (IL1R1). Furthermore, IL36G also can function as an agonist of NFκB activation through the orphan IL1-receptor-related protein 2. Human IL36G (152 a.a.) shares 58%, 59%, 68% and 69% amino acid sequence identity with mouse, rat, bovine and equine IL36G, respectively, and 23-57% amino acid sequence identity with other family members.

Product Specifications

Catalog ID 1110-36F
MW ~17.0 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1110-36F-10 3-4 Weeks 10 μg $ 165.00
1110-36F-100 3-4 Weeks 100 μg $ 1,315.00
1110-36F-500 3-4 Weeks 500 μg $ 2,535.00

IL36G (IL-36 gamma), Human (169 a.a.)

Catalog ID Size Pricing
1110-36E-10 10 μg $ 165.00

Description

Interleukin-36G (IL36G) is a pro-inflammatory cytokine that plays an important role in the pathophysiology of several diseases. IL36A, IL36B and IL36G; (formerly IL1F6, IL1F8, and IL1F9) are IL1 family members that signal through the IL1 receptor family members IL1Rrp2 (IL1RL2) and IL1RAcP. IL36B is secreted when transfected into 293-T cells and could constitute part of an independent signaling system analogous to that of IL1A and IL1B receptor agonist and interleukin-1 receptor type I (IL1R1). Furthermore, IL36G also can function as an agonist of NFκB activation through the orphan IL1-receptor-related protein 2. Recombinant human IL36G is synthesized as a protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site.There is a 53% amino acid homology between human and mouse IL36G. IL36G also has a 25-55% amino acid homology with IL36G and IL1RN, IL1B, IL36RN, IL36A, IL37, IL36B and IL1F10.

Product Specifications

Catalog ID 1110-36E
MW ~18.7 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
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1110-36E-10 3-4 Weeks 10 μg $ 165.00

IL36RN (IL-36RA), Human

Catalog ID Size Pricing
1110-36G-5 5 μg $ 115.00
1110-36G-100 100 μg $ 745.00
1110-36G-500 500 μg $ 1,539.00

Description

Interleukin-36 receptor antagonist (IL36RN) is a secreted protein which belongs to the interleukin 1 cytokine family (IL1 family) and is predominantly expressed in keratinocytes but not in fibroblasts, endothelial cells or melanocytes. IL36RN has been reported to antagonize the biological activity of IL36A (IL1F6), IL36B (IL1F8), and IL36G (IL1F9). Furthermore, it could constitute part of an independent signaling system analogous to IL1A, IL1B receptor agonist and interleukin-1 receptor type I (IL1R1). However, the receptor for IL36RN has not been positively identified. Indirect evidence suggests that IL1Rrp2 is its receptor. Recombinant human IL36RN contains 154 amino acid residues and shares 91% amino acid sequence identity with murine IL36RN.

Product Specifications

Catalog ID 1110-36G
MW ~16.8 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1110-36G-5 3-4 Weeks 5 μg $ 115.00
1110-36G-100 3-4 Weeks 100 μg $ 745.00
1110-36G-500 3-4 Weeks 500 μg $ 1,539.00

IL36RN (IL-36RA), Murine

Catalog ID Size Pricing
1310-36G-10 10 μg $ 165.00
1310-36G-100 100 μg $ 745.00
1310-36G-500 500 μg $ 1,539.00

Description

Interleukin-36 receptor antagonist (IL36RN) is a secreted protein that belongs to the interleukin 1 cytokine family (IL1 family) and is predominantly expressed in keratinocytes, but not in fibroblasts, endothelial cells or melanocytes. IL36RN has been reported to antagonize the biological activity of IL36A (IL1F6), IL36B (IL1F8), and IL36G (IL1F9). Furthermore, it could constitute part of an independent signaling system analogous to IL1A, IL1B receptor agonist and interleukin-1 receptor type I (IL1R1). In addition, the receptor for IL36RN has not been positively identified; however there is indirect evidence that suggests IL1Rrp2 is the receptor. Recombinant murine IL36 RN contains 154 amino acid residues and shares 91% amino acid sequence identity with human IL36RN.

Product Specifications

Catalog ID 1310-36G
MW ~16.9 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1310-36G-10 3-4 Weeks 10 μg $ 165.00
1310-36G-100 3-4 Weeks 100 μg $ 745.00
1310-36G-500 3-4 Weeks 500 μg $ 1,539.00

IL4, Human

Catalog ID Size Pricing
1110-04-5 5 μg $ 115.00
1110-04-100 100 μg $ 795.00
1110-04-500 500 μg $ 1,629.00

Description

Interleukin 4 is a pleiotropic cytokine that regulates diverse T-cell and B-cell responses, including cell proliferation, survival and gene expression. Produced by mast cells, T-cells and bone marrow stromal cells, IL4 regulates the differentiation of naive CD4+ T-cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL4, IL5, IL6, IL10, and IL13, which favor a humoral immune response. Another dominant function of IL4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL4 production by Th2 cells has been associated with elevated IgE production and allergy.

Product Specifications

Catalog ID 1110-04
MW ~15.0 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1110-04-5 In stock 5 μg $ 115.00
1110-04-100 3-4 Weeks 100 μg $ 795.00
1110-04-500 3-4 Weeks 500 μg $ 1,629.00

IL4, Murine

Catalog ID Size Pricing
1310-04-5 5 μg $ 115.00
1310-04-100 100 μg $ 795.00
1310-04-500 500 μg $ 1,629.00

Description

Interleukin 4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL4, IL5, IL6, IL10, and IL13, which favor a humoral immune response. Another dominant function of IL4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL4 production by Th2 cells has been associated with elevated IgE production and allergy.

Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.

Product Specifications

Catalog ID 1310-04
MW ~13.5 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1310-04-5 In stock 5 μg $ 115.00
1310-04-100 3-4 Weeks 100 μg $ 795.00
1310-04-500 3-4 Weeks 500 μg $ 1,629.00

IL4, Rat

Catalog ID Size Pricing
1510-04-5 5 μg $ 115.00

Description

Interleukin-4 (IL4) is a pleiotropic cytokine that induces differentiation of naive helper T-cells (Th0 cells) to Th2-cells, and is produced by mast cells, activated T-cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2-cells. IL4 can enhance both the secretion and cell surface expression of IgE and IgG1. IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The rat IL4 shares about 40-45% amino acid sequence identity with human, mouse, rhesus macaque IL4, and they are species-specific in their activities.

Product Specifications

Catalog ID 1510-04
MW ~14.0 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1510-04-5 3-4 Weeks 5 μg $ 115.00

IL4, Rhesus macaque

Catalog ID Size Pricing
1210-04-5 5 μg $ 115.00

Description

Interleukin-4 (IL4) is a pleiotropic cytokine mainly produced by mast cells, activated T-cells and bone marrow stromal cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Additionally, IL4 enhances both the secretion and expression of cell surface IgE and IgG1. IL4 also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. The rhesus macaque IL4 shares about 40-45% amino acid sequence identity with human, mouse, and rat IL4 and is species-specific in its activities.

Product Specifications

Catalog ID 1210-04
MW ~15.0 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1210-04-5 3-4 Weeks 5 μg $ 115.00

IL5, Human

Catalog ID Size Pricing
1110-05-10 10 μg $ 165.00

Description

The IL5 gene is located on chromosome 5 in humans, in close proximity to the genes encoding IL3, IL4, and granulocyte-macrophage colony-stimulating factor (CSF2), which are often co-expressed in TH2 cells. Through binding to the IL5 receptor, IL5 stimulates B cell growth and increases immunoglobulin secretion and is also a key mediator in eosinophil activation. Interleukin 5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. Human IL5 is 115 amino acids long (murine IL5 has 133 amino acids) and is a TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL3 and CSF2), this glycoprotein is a homodimer in its active form.

Product Specifications

Catalog ID 1110-05
MW ~26.5 kDa
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
1110-05-10 3-4 Weeks 10 μg $ 165.00

IL5, Murine

Catalog ID Size Pricing
1310-05-5 5 μg $ 115.00
1310-05-100 100 μg $ 745.00
1310-05-500 500 μg $ 1,539.00

Description

The IL5 gene is located on chromosome 11 in mice, in close proximity to the genes encoding IL3, IL4, and granulocyte-macrophage colony-stimulating factor (CSF2), which are often co-expressed in TH2 cells. Through binding to the IL5 receptor, IL5 stimulates B cell growth and increases immunoglobulin secretion and is also a key mediator in eosinophil activation. Interleukin 5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. Murine IL5 is a TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL3 and CSF2), this glycoprotein is a homodimer in its active form.

Product Specifications

Catalog ID 1310-05
MW ~26.2 kDa
Storage/Handling Store desiccated at -20°C.
View
Packs & Pricing
,
1310-05-5 3-4 Weeks 5 μg $ 115.00
1310-05-100 3-4 Weeks 100 μg $ 745.00
1310-05-500 3-4 Weeks 500 μg $ 1,539.00

IL5, Rat

Catalog ID Size Pricing
1510-05-10 10 μg $ 165.00

Description

The IL5 gene is located on chromosome 11 in rats, in close proximity to the genes encoding IL3, IL4, and granulocyte-macrophage colony-stimulating factor (CSF2), which are often co-expressed in TH2 cells. Through binding to the IL5 receptor, IL5 stimulates B cell growth and increases immunoglobulin secretion and is also a key mediator in eosinophil activation. Interleukin 5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. Rat IL5 is a TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL3 and CSF2), this glycoprotein is a homodimer in its active form.

Product Specifications

Catalog ID 1510-05
MW ~26.2 kDa
Storage/Handling Store desiccated at -20°C.
View
Packs & Pricing
,
1510-05-10 3-4 Weeks 10 μg $ 165.00

IL6, Human

Catalog ID Size Pricing
1110-06-5 5 μg $ 115.00
1110-06-100 100 μg $ 745.00
1110-06-500 500 μg $ 1,539.00

Description

Interleukin-6 (IL6) is encoded by theIL6 gene in humans and acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T-cells and macrophages to stimulate an immune response. Furthermore, it plays an essential role in the final differentiation of B-cells into Ig-secreting cells, which are involved in lymphocyte and monocyte differentiation. IL6 also induces myeloma and plasmacytoma growth. The human IL6 is a single nonglycosylated polypeptide chain containing 183 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL6RG chain (CD126), and the signal- transducing component gp130 (also called CD130). The human IL6 is equally active on mouse and rat cells.

Sterile Filtered White Lyophilized Powder. Purified and tested for use in cell culture.

Product Specifications

Catalog ID 1110-06
MW ~20.7 kDa
Storage/Handling Store desiccated at -20°C.
View
Packs & Pricing
,
1110-06-5 3-4 Weeks 5 μg $ 115.00
1110-06-100 3-4 Weeks 100 μg $ 745.00
1110-06-500 3-4 Weeks 500 μg $ 1,539.00

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