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Showing 21-26 of 26 items.

Proteinase K Solution 20 mg/ml

Catalog ID Size Pricing
P-480-SL2 2 mL $ 49.00
P-480-SL4 4 mL (2 x 2 mL) $ 79.00
P-480-SL10 10 mL $ 159.00

Description

Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.

Product Specifications

Catalog ID P-480-SL
CAS # 39450-01-6
MW 28.5 kDa
Grade MOLECULAR BIOLOGY GRADE
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P-480-SL2 In stock 2 mL $ 49.00
P-480-SL4 In stock 4 mL (2 x 2 mL) $ 79.00
P-480-SL10 In stock 10 mL $ 159.00

Proteinase K, RNase/DNase free

Catalog ID Size Pricing
P-480-100 100 mg $ 54.00
P-480-500 500 mg $ 173.00
P-480-1 1 g $ 215.00
P-480-2 2 x 1 g $ 415.00
P-480-3 3 x 1 g $ 615.00
P-480-4 4 x 1 g $ 810.00
P-480-5 5 x 1 g $ 1,005.00

Description

Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.

Product Specifications

Catalog ID P-480
Name(s) Proteinase K, RNase/DNase free
CAS # 39450-01-6
Grade MOLECULAR BIOLOGY GRADE
Activity ≥30 U/mg
MW 28.5 kDa
Source Yeast cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease.
Storage/Handling Store at -20°C.
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Packs & Pricing
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P-480-100 In stock 100 mg $ 54.00
P-480-500 In stock 500 mg $ 173.00
P-480-1 In stock 1 g $ 215.00
P-480-2 In stock 2 x 1 g $ 415.00
P-480-3 In stock 3 x 1 g $ 615.00
P-480-4 In stock 4 x 1 g $ 810.00
P-480-5 In stock 5 x 1 g $ 1,005.00

TCEP HCl

Catalog ID Size Pricing
TCEP1 1 g $ 29.00   $ 23.20
TCEP2 2 g $ 49.00   $ 39.20
TCEP5 5 g $ 89.00   $ 71.20
TCEP10 10 g $ 145.00   $ 116.00
TCEP25 25 g $ 289.00   $ 231.20
TCEP50 50 g $ 529.00   $ 423.20
TCEP100 100 g $ 1,035.00   $ 828.00

Description

TCEP-HCl is an odorless reducing agent suitable for protein applications and in the preparation for electrophoresis. TCEP is water soluble, is stable in a variety of solutions and is particularly useful in the labeling of cysteine residues with maleimides, preventing the cysteines from forming disulfide bonds. As a reducing agent, TCEP has many other desirable features.

Product Specifications

Catalog ID TCEP
Name(s) TCEP-HCl;
Tris (2-Carboxyethyl) phosphine Hydrochloride
CAS # 51805-45-9
Grade Molecular Biology Grade
Formula C9H15O6P • HCl
MW 286.65 g/mol
PubChem Chemical ID 2734570
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
,
TCEP1 In stock 1 g $ 29.00
$ 23.20
TCEP2 In stock 2 g $ 49.00
$ 39.20
TCEP5 In stock 5 g $ 89.00
$ 71.20
TCEP10 In stock 10 g $ 145.00
$ 116.00
TCEP25 In stock 25 g $ 289.00
$ 231.20
TCEP50 In stock 50 g $ 529.00
$ 423.20
TCEP100 In stock 100 g $ 828.00

Tris (Tris Base)

Catalog ID Size Pricing
T-400-500 500 g $ 49.00   $ 39.20
T-400-1 1 kg $ 79.00   $ 63.20
T-400-5 5 kg $ 279.00   $ 223.20
T-400-10 10 kg $ 545.00

Description

GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0 and is also used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, it can also be used for many custom running and loading buffers, most often with glycine and SDS.

Product Specifications

Catalog ID T-400
Name(s) Tris, Tris Buffer;
2-Amino-2-(hydroxymethyl)-1,3-propanediol
CAS # 77-86-1
Formula C4H11NO3
MW 121.14 g/mol
Grade ULTRA PURE GRADE
Storage/Handling Store desiccated at room temperature.
PubChem Chemical ID 6503
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Packs & Pricing
,
T-400-500 In stock 500 g $ 49.00
$ 39.20
T-400-1 In stock 1 kg $ 79.00
$ 63.20
T-400-5 In stock 5 kg $ 279.00
$ 223.20
T-400-10 In stock 10 kg $ 545.00

Vancomycin Hydrochloride, USP Grade

Catalog ID Size Pricing
V-200-1 1 g $ 65.00
V-200-5 5 g $ 145.00
V-200-25 25 g $ 345.00

Description

Vancomycin hydrochloride is a member of the glycopeptide antibiotic class derived from Streptomyces orientalis. It is effective against gram-positive bacteria and used in studies of antibiotic resistance bacteria and nanoparticle transport studies. Exhibiting low toxicity to plants, vancomycin is suitable for plant selection and prevention of bacterial contamination in plant cell culture applications.

Product Specifications

Catalog ID V-200
CAS # 1404-93-9
MW 1485.71 g/mol
Storage/Handling Store desiccated at -20°C.
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Packs & Pricing
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V-200-1 In stock 1 g $ 65.00
V-200-5 In stock 5 g $ 145.00
V-200-25 In stock 25 g $ 345.00

X-Gal

Catalog ID Size Pricing
X4281C 1 g $ 59.00
X4281C10 10 g $ 289.00

Description

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.

X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity. Bacteria cells that retain active β-galactosidase will result in characteristic blue colonies. Successful disruption of the lacZα gene disrupts the α-complementation of the β-galactosidase gene and the precipitate does not form, resulting in white colonies.

Many other applications also use X-Gal as a substrate to detect β-galactosidase activity. These include β-galactosidase -antibody linked immunoassays and immunohistochemistry, coliphage detection based on β-galactosidase induction and the detection of micrometastasis formation during tumor progession.

Product Specifications

Catalog ID X4281
Name(s) X-Gal;
5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside
CAS # 7240-90-6
Grade MOLECULAR BIOLOGY GRADE
Formula C14H15BrClNO6
MW 408.63 g/mol
Biological Activity Blue color with Lambda, pUC, and M13
PubChem Chemical ID 619018
Storage/Handling Store at -20°C. Protect from light.
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Packs & Pricing
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X4281C In stock 1 g $ 59.00
X4281C10 In stock 10 g $ 289.00

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