Enzymes
Alkaline Phosphatase, 2-Component System
Catalog ID | Size | Pricing | |
---|---|---|---|
A-440-1 | 1 KU | $ 146.00 |
Description
Alkaline phosphatase is an enzyme commonly used for the removal of terminal phosphates from the 5' end of DNA or RNA strands to prevent reannealing of the cohesive ends and facilitate future radiolabeling of the strands.
This system includes 30 U/μL of alkaline phosphatase in a 50% glycerol solution and a dilution buffer that allows for the preparation of a 3 U/μL solution for use in DNA modification.
Product Specifications
Catalog ID | A-440 |
---|---|
Grade | BIOTECHNOLOGY GRADE |
Storage/Handling | store at 4°C. Do NOT freeze. |
Cellulase R-10
Catalog ID | Size | Pricing | |
---|---|---|---|
C8001.0001 | 1 g | $ 105.00 | |
C8001.0005 | 5 g | $ 225.00 | |
C8001.0010 | 10 g | $ 319.00 |
Description
Cellulase R-10, also called Cellulase "Onozuka" R-10, is derived from Trichoderma Viride. Cellulase R-10 is a multi-enzymatic system consisting of various enzymes, including cellulase, hemicellulase, pectinase and protease. Cellulase R-10 is typically used in association with macerozyme R-10 in the decomposition of plant cell walls to produce protoplasts.
Product Specifications
Catalog ID | C8001 |
---|---|
CAS # | 9012-54-8 |
Storage/Handling | Store at room temperature. |
Cellulase RS
Catalog ID | Size | Pricing | |
---|---|---|---|
C8003.0001 | 1 g | $ 155.00 | |
C8003.0005 | 5 g | $ 449.00 | |
C8003.0010 | 10 g | $ 705.00 |
Description
Cellulase “Onozuka RS” is produced by a mutant Trichoderma viride that was derived from the parent strain for Cellulase “Onozuka -R-10”. Cellulase RS contains a very high activity of decomposing natural celluloses. This type of cellulase can be used to obtain protoplasts in a very short time and dissolves cell walls of a wider range of plants.
Product Specifications
Catalog ID | C8003 |
---|---|
CAS # | 9012-54-8 |
Storage/Handling | Store desiccated at 4°C. |
DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg
Catalog ID | Size | Pricing | |
---|---|---|---|
D-301-50 | 50 mg | $ 89.00 | |
D-301-100 | 100 mg | $ 125.00 | |
D-301-500 | 500 mg | $ 505.00 | |
D-301-1 | 1 g | $ 965.00 |
Description
DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.
Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and a phosphate group at the 5' position. The optimum pH is 7.8.
DNase I is activated by bivalent metals and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca2+.
Product Specifications
Catalog ID | D-301 |
---|---|
CAS # | 9003-98-9 |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store desiccated at -20°C. |
DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg
Catalog ID | Size | Pricing | |
---|---|---|---|
D-303-100 | 100 mg | $ 45.00 | |
D-303-500 | 500 mg | $ 115.00 | |
D-303-1 | 1 g | $ 209.00 | |
D-303-5 | 5 g | $ 835.00 |
Description
DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.
Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.
DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca 2+.
Product Specifications
Name | DNase I |
---|---|
CAS # | 9003-98-9 |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store desiccated at -20°C. |
DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg
Catalog ID | Size | Pricing | |
---|---|---|---|
D-300-100 | 100 mg | $ 55.00 | |
D-300-500 | 500 mg | $ 145.00 | |
D-300-1 | 1 g | $ 255.00 | |
D-300-5 | 5 g | $ 1,019.00 |
Description
DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.
Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.
DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+.
Product Specifications
Catalog ID | D-300 |
---|---|
CAS # | 9003-98-9 |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store desiccated at -20°C. |
Glucose Oxidase >100 U/mg
Catalog ID | Size | Pricing | |
---|---|---|---|
G-040-50 | 50 KU | $ 66.00 | |
G-040-250 | 250 KU | $ 336.00 | |
G-040-1 | 1 MU | $ 827.00 |
Description
Glucose oxidase comes from Aspergillus niger and has many applications. Glucose oxidase can be used in the determination of glucose in colorimetric assays in conjunction with peroxidase, in the determination of glucose biosensors or for use as an enzyme label.
Product Specifications
Catalog ID | G-040 |
---|---|
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at -20°C. |
Glucose Oxidase >225 U/mg
Catalog ID | Size | Pricing | |
---|---|---|---|
G-041-50 | 50 KU | $ 78.00 | |
G-041-250 | 250 KU | $ 371.00 |
Description
Glucose oxidase comes from Aspergillus niger and has many applications. Glucose oxidase can be used in the determination of glucose in colorimetric assays in conjunction with peroxidase, in the determination of glucose biosensors or for use as en enzyme label.
Product Specifications
Catalog ID | G-041 |
---|---|
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at -20°C. |
Horseradish peroxidase (HRP)
Catalog ID | Size | Pricing | |
---|---|---|---|
P-100-100 | 100 mg | $ 96.00 | |
P-100-250 | 250 mg | $ 178.00 | |
P-100-500 | 500 mg | $ 294.00 | |
P-100-1 | 1 g | $ 504.00 |
Description
Horseradish peroxidase (HRP) is the most commonly used antibody-conjugated enzyme. Substrates for HRP are luminol and benzidine substrates. Tetramethylbenzidine (TMB) and similar substrates are oxidized by HRP in the presence of hydrogen peroxide, or nontoxic substitutes, to yield a chinoid structure of intense blue color.
Product Specifications
Catalog ID | P-100 |
---|---|
CAS # | 9003-99-0 |
MW | ~40,000 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at -20°C. |
Lysozyme, Egg White
Catalog ID | Size | Pricing | |
---|---|---|---|
L-040-1 | 1 g | $ 29.00 | |
L-040-5 | 5 g | $ 59.00 | |
L-040-10 | 10 g | $ 85.00 | |
L-040-25 | 25 g | $ 145.00 | |
L-040-100 | 100 g | $ 279.00 | |
L-040-250 | 250 g | $ 635.00 |
Description
Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between the N-acetylmuramic acid and the N-acetyl-D-glucosamine residues in peptidoglycan.
Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence of EDTA.
Product Specifications
Catalog ID | L-040 |
---|---|
Name(s) | Lysozyme, Egg White |
CAS # | 12650-88-3 |
Grade | MOLECULAR BIOLOGY GRADE |
Activity | >45,000 FIP U/mg |
Storage/Handling | Store at -20°C. |
Macerozyme R-10
Catalog ID | Size | Pricing | |
---|---|---|---|
M8002.0001 | 1 g | $ 105.00 | |
M8002.0005 | 5 g | $ 225.00 | |
M8002.0010 | 10 g | $ 319.00 |
Description
Macerozyme R-10 is a macerating enzyme from the Rhizopus sp. which is perfectly suited for the isolation of plant cells. Macerozyme R-10 is often used in combination with Cellulase "Onozuka R-10" in the isolation of plant protoplasts.
Product Specifications
Catalog ID | M8002 |
---|---|
CAS # | 9032-75-1 |
Storage/Handling | Store at 4°C. |
Pectolyase Y-23
Catalog ID | Size | Pricing | |
---|---|---|---|
P8004.0001 | 1 g | $ 459.00 | |
P8004.0005 | 5 g | $ 1,799.00 |
Description
Product Specifications
Catalog ID | P8004 |
---|---|
CAS # | 9033-35-6 |
Proteinase K Solution 20 mg/ml
Catalog ID | Size | Pricing | |
---|---|---|---|
P-480-SL2 | 2 mL | $ 49.00 | |
P-480-SL4 | 4 mL (2 x 2 mL) | $ 79.00 | |
P-480-SL10 | 10 mL | $ 159.00 |
Description
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.
Product Specifications
Catalog ID | P-480-SL |
---|---|
CAS # | 39450-01-6 |
MW | 28.5 kDa |
Grade | MOLECULAR BIOLOGY GRADE |
Proteinase K, RNase/DNase free
Catalog ID | Size | Pricing | |
---|---|---|---|
P-480-100 | 100 mg | $ 54.00 | |
P-480-500 | 500 mg | $ 173.00 | |
P-480-1 | 1 g | $ 215.00 | |
P-480-2 | 2 x 1 g | $ 415.00 | |
P-480-3 | 3 x 1 g | $ 615.00 | |
P-480-4 | 4 x 1 g | $ 810.00 | |
P-480-5 | 5 x 1 g | $ 1,005.00 |
Description
Proteinase K is a highly reactive nonspecific serine protease that belongs to the subtilisin family of proteins. It cleaves at the carboxylic acid side of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K is capable of inactivating RNases and DNases and is used in the isolation or preparation of high molecular weight nucleic acids. Proteinase K is also useful for helping to characterize enzymes, due to its cleavage specificity. This enzyme was designated proteinase K because of its ability to hydrolyze keratin. Proteinase K is stable in a wide variety of detergents and buffer salts and at various temperatures and pH. The isoelectric point of proteinase K is 8.9.
Product Specifications
Catalog ID | P-480 |
---|---|
Name(s) | Proteinase K, RNase/DNase free |
CAS # | 39450-01-6 |
Grade | MOLECULAR BIOLOGY GRADE |
Activity | ≥30 U/mg |
MW | 28.5 kDa |
Source | Yeast cells with cloned gene encoding Engyodontium album (Tritirachium album) endolytic protease. |
Storage/Handling | Store at -20°C. |
RNase A, >50 U/mg
Catalog ID | Size | Pricing | |
---|---|---|---|
R-050-50 | 50 mg | $ 55.00 | |
R-050-100 | 100 mg | $ 95.00 | |
R-050-500 | 500 mg | $ 215.00 | |
R-050-1 | 1 g | $ 319.00 | |
R-050-5 | 5 g | $ 1,379.00 | |
R-050-10 | 10 g | $ 2,685.00 |
Description
RNase A, isolated from bovine pancreas, degrades single-stranded RNA at the C and U residues; therefore, RNase A is pyrimidine specific. Degradation occurs by cleaving the phosphodiester bond located between the 5’-ribose of the nucleotide and the phosphate group that is connected to the 3’-ribose of the neighboring pyrimidine.
Product Specifications
Catalog ID | R-050 |
---|---|
CAS # | 9001-99-4 |
MW | 13.7 kDa |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at -20°C. |
Trypsin 1:250
Catalog ID | Size | Pricing | |
---|---|---|---|
T-161-25 | 25 g | $ 65.00 | |
T-161-50 | 50 g | $ 105.00 | |
T-161-100 | 100 g | $ 175.00 | |
T-161-250 | 250 g | $ 355.00 |
Description
Trypsin is a serine protease found in the digestive system of many vertebrates where it hydrolyzes proteins. Through trypsinisation, it cleaves peptides at the carboxyl side of arginine and lysine, unless they are followed by a proline.
Trypsin has an optimal operating pH of about 7.5-8.5 and optimal operating temperature of about 37°C. Unlike chymotrypsin, the activity of trypsin is not affected by the inhibitor tosyl phenylalanyl chloromethyl ketone (TPCK).
Product Specifications
Catalog ID | T-161 |
---|---|
CAS # | 9002-07-7 |
Grade | TISSUE CULTURE GRADE |
Storage/Handling | Store at -20°C. |
Trypsin 1:300
Catalog ID | Size | Pricing | |
---|---|---|---|
T-162-25 | 25 g | $ 85.00 | |
T-162-100 | 100 g | $ 245.00 |
Description
Trypsin is a serine protease found in the digestive system of many vertebrates where it hydrolyzes proteins. Through trypsinisation, it cleaves peptides at the carboxyl side of arginine and lysine, unless they are followed by a proline.
Trypsin has an optimal operating pH of about 7.5-8.5 and optimal operating temperature of about 37°C. Unlike chymotrypsin, the activity of trypsin is not affected by the inhibitor tosyl phenylalanyl chloromethyl ketone (TPCK).
Trypsin 1:300 indicates that one part trypsin will digest 300 parts casein in the USP activity assay.
Product Specifications
Catalog ID | T-162 |
---|---|
CAS # | 9002-07-7 |
Grade | TISSUE CULTURE GRADE |
Storage/Handling | Store at -20°C. |
Trypsin >2500 U/mg, USP Grade
Catalog ID | Size | Pricing | |
---|---|---|---|
T-160-1 | 1 g | $ 59.00 | |
T-160-5 | 5 g | $ 219.00 | |
T-160-25 | 25 g | $ 865.00 |
Description
Trypsin is a serine protease found in the digestive system of many vertebrates where it hydrolyzes proteins. Through trypsinisation, it cleaves peptides at the carboxyl side of arginine and lysine, unless they are followed by a proline.
Trypsin has an optimal operating pH of about 7.5-8.5 and optimal operating temperature of about 37°C. Unlike chymotrypsin, the activity of trypsin is not affected by the inhibitor tosyl phenylalanyl chloromethyl ketone (TPCK).
Product Specifications
Catalog ID | T-160 |
---|---|
CAS # | 9002-07-7 |
Storage/Handling | Store desiccated at -20°C. |