Enzyme Substrates

4-MU-beta-D-GlcNAc (4-Methylumbelliferyl-2- acetamido-2-deoxy- β- glucopyranoside) is used as a fluorogenic substrate for N-Acetyl-beta-D-glucosaminidase (NAGase), yielding a blue fluorescent solution.

NAGase is an enzyme abundantly found in the renal tubular epithelia. Increased amounts of this enzyme in urine is often associated with various renal diseases. Therefore, interest in measuring its activity using 4-Methylumbelliferyl-2- acetamido-2-deoxy- β- glucopyranoside in order to diagnose renal diseases has grown more popular.

4-Methylumbelliferyl-N- acetyl-β-D- galactoside is a fluorogenic substrate for N-acetyl-beta-D-galactosaminidase and can be used for the detection of Candida albicans.

4-Methylumbelliferyl-α- L-iduronic acid cyclohexylammonium salt is a fluorogenic soluble enzyme substrate for α-L-iduronidase. α-L-iduronidase is deficient in the genetic disorder Mucopolysaccharidosis type I, also called Hurler syndrome. 4-Methylumbelliferyl-α-L-iduronide is used in the diagnosis of Hurler syndrome as the substrate in an enzymatic assay of α-L-iduronidase activity.

4-Methylumbelliferyl-α-D-glucopyranoside is a fluorogenic substrate for the identification, characterization and kinetic analysis of α-glucosidase activity. It is routinely used as a substrate for the assay of acid α-glucosidase in the diagnosis of Pompe's disease.

4-Methylumbelliferyl-β-D-cellobioside acts as a fluorogenic substrate used to measure the activity of (1,4)-β-Glucanase.

(1,4)-β-Glucanase is an enzyme that breaks down glucose by disrupting the glycosidic bonds. This enzyme is also involved in the cell wall synthesis for various fungi.

4-Methylumbelliferyl-β-D-cellobioside can also be used in Cel D Fusion screenings and activity staining in polyacrylamide gels, yielding blue fluorescence as a result.

Fluorogenic substrate for β-D-galactosidase, yielding a blue fluorescent solution upon cleavage.

4-Methylumbelliferyl-β- D-mannopyranoside is a fluorogenic substrate for detection of β-D-mannopyranosidase.

4-Nitrophenyl phosphate, disodium salt hexahydrate is a chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases and is used for determining alkaline phosphatase activity in human serum.

4-Nitrophenyl-N-acetyl- β-D-galactosaminide
p-Nitrophenyl-2-acetamido-2-deoxy-β-D-galactoside; PNP-β-D-GalNAc

4-Nitrophenyl-N-acetyl-β- D-glucosaminide is a chromogenic β-Glucosaminidase substrate, yielding a yellow solution upon cleavage, used particularly in yeasts and molds.

4-Nitrophenyl-α-D- galactopyranoside is a chromogenic substrate yielding a soluble chromophor for the colorimetric assay of alpha-D-galactosidase upon cleavage. Used for the detection of hydrolases in tissues and membranes.

4-Nitrophenyl-α-D- glucopyranoside is a chromogenic substrate for alpha-D-glucosidase, yielding a yellow solution upon cleavage. 4-Nitrophenyl-α-D- glucopyranoside is also used for the detection of glucansucrases and for yeast alpha-D-glucosidase and for crystal structure studies of the complexes of concanavalin A.

4-Nitrophenyl-α-D- maltohexaoside is a chromogenic substrate for α-amylase, yielding a yellow solution upon cleavage.

Chromogenic substrate, yielding a soluble yellow chromophor for the colorimetric assay of α-L-fucosidase, especially in human serum.