D_Products

EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making the dye readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen® dye is nonmutagenic and noncytotoxic by being completely impermeable to cell membranes, making it a great alternative to SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer (Ohta, et al. (2001). Mutation Research, 492(1), 91-97).

The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.

GoldBio’s GB10B-Pro™ Electrocompetent E. coli cells are especially designed for the most demanding cloning applications. GB10B-Pro™ cells will provide the greatest number of transformants for when your research requires it, including assembling large and multi-DNA fragments, cloning large (≥10 kb up to 350 kb) or difficult construct transformations, working with synthetic bio-applications, and even BAC cloning.

Product Specifications
Competent cell type: ElectroCompetent
Equivalent to: DH10B
Species: E. coli
Transformation efficiency:≥1 x 10⁹ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s GB10B™ Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. GB10B™ Chemically Competent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.

Product Specifications
Competent cell type: Chemically competent
Equivalent to: DH10B
Species: E. coli
Transformation efficiency: ≥8.2 x 10⁶ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s GB10B™ Electrocompetent E. coli cells are high efficiency cells, ≥2 x 10⁸ cfu/µg plasmid DNA, suitable for a wide variety of applications requiring high transformation efficiencies, such as cloning and subcloning, as well as working with either cDNA or gDNA library construction. GB10B™ Electrocompetent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine. Here, we present a detailed protocol for electroporation using GB10B™ Electrocompetent E. coli cells.

Product Specifications
Competent cell type: ElectroCompetent
Equivalent to: DH10B
Species: E. coli
Transformation efficiency: ≥2 x 10⁸ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s GB5-alpha™ Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.

Product Specifications
Competent cell type: Chemically Competent
Equivalent to: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 10⁶ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s GB5-alpha™ Electrocompetent E. coli are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. Plasmid quality and yields are both enhanced in our GB5-alpha™ cells due to mutations in both endA1 and recA1.

Product Specifications
Competent cell type: ElectroCompetent
Equivalent to: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 10⁸ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s Illumination™ Dura-Luc Lyophilized Firefly HTS Assay Kit contains a proprietary formulation that modifies and stabilizes the enzymatic reaction to produce a long lasting bioluminescent signal by preventing the formation of adenyl-oxyluciferin at the enzyme surface. The Dura-Luc system is a highly sensitive and homogenous luciferase reporter assay that has a signal half-life of three hours for the best quantification of expression in mammalian cell lines, allowing for batch processing and plate to plate comparisons in high-throughput screening experiments.

Firefly Luciferase Assays are one of the leading reporter assays in the world in the measurement of gene function and gene regulation as well as being widely used in pharmaceutical screening. The luciferase assays are sensitive and convenient due to the absence of endogenous luciferase activity in most cell types and tissue. Firefly luciferase is a monomeric 62 kDa protein typically isolated from the firefly, Photinus pyralis, which catalyzes the ATP-dependent D-luciferin in the presence of oxygen and Mg2+ to oxyluciferin producing a yellow to greenish light (~560 nm).

The Dura-Luc Lyophilized Firefly Kit contains an assay buffer that is provided in a lyophilized form for economical shipping and more convenient storage. A reconstitution buffer is also provided with this kit.

The primary attribute of DDM is its capability to extract hydrophobic proteins while maintaining the solution-phase protein conformation. DDM also allows the protein to be reformed following denaturization. Most membrane-bound proteins are α-helix bundles, which are less robust to changes in their cellular environment. DDM offers a detergent capable of preserving these proteins. This is in contrast to other, harsher ionic detergents that destabilize and permanently denature α-helical membrane-bound proteins. DDM has been used to solubilize the multidrug-transporter protein, EmrE, as well as the nucleoside-specific porin protein, Tsx.

n-Dodecyl-beta-D-glucopyranoside is a sugar based detergent and reagent for chemical synthesis.

Neutral red dye is a dark brown to dark green power used to stain nuclei red, in cell viability tests, and as a pH indicator (transition of pH indicator: pH 6.8-8.0, red to yellow). Additionally, it is used to prepare neutral red paper and as a histological stain for lysozymes.

GoldBIo Orange 6x DNA Loading Dye is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains one dye (Orange G) for tracking the DNA migration.

The Orange G migrates at approximately 50 bp on a standard 1% TAE agarose gel (30 bp on 2% TAE agarose gel). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Composition

• 0.15% Orange G
• 10mM Tris-HCl (pH 8.0)
• 60% glycerol
• 60mM EDTA

Dimethylsulfoxide (DMSO) is a very polar organic compound that is useful in organic chemistry for solubilizing organic and inorganic compounds. DMSO is also used as a cryoprotectant for cell media, preventing the formation of ice crystals which would otherwise damage cells. DMSO can be used in PCR reactions to inhibit the self-complementation of DNA or PCR primers and increase amplification, especially for DNA sequences which have GC rich sequences.

GoldBio's DMSO is filter sterilized through a 0.2 μg filter. Sterile DMSO is ideal for working with tissue culture or cell culture in which there is a need for eliminating all potential sources of contamination.

GoldBio's SuperLadder (50 bp-25 kb) is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder is composed of 26 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100 and 50 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA.

This product contains four enhanced bands (3k, 1.2k, 500 and 200 bp) for easier reference. In addition, three tracking dyes are present (Xylene cyanol FF, Bromophenol blue and Orange G) which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA respectively during electrophoresis on a 1% TAE agarose gel which helps in real time monitoring.

GoldBio Tri-Color 6x DNA Loading Dye is a pre-mixed buffer for tracking DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue and Orange G) for tracking the DNA migration.

The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively (800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively). Glycerol is used to keep the DNA at the bottom of the well. The presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Composition

• 0.03% Xylene cyanol FF
• 0.03% Bromophenol blue
• 0.15% Orange G
• 10mM Tris-HCl (pH 8.0)
• 60% glycerol
• 60mM EDTA

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 19 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-10,000 base pairs. The 500, 1.5K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.