D_Products

3,5-Dichloro-2-hydroxybenzenesulfonic acid, disodium salt is a reagent used to produce peroxides when combined with 4-aminoantipyrine. It is also used in quantification of hydrogen peroxide through horseradish peroxidase-mediated conversion of itself and 4-aminoantipyrine to a colored product. Under this principle, it is used to determine uric acid in serum as oxidation of uric acid in a uricase-catalyzed reaction yields hydrogen peroxide.

3,5-Diiodo-L-tyrosine dehydrate is an a substrate in the assay of halogenated tyrosine and thyroid hormones aminotransferase. It is also an intermediate in the synthesis and pathways of thyroid hormone metabolism.

4'-Demethylepipodophyllotoxin is an inhibitor of microtubule assembly.

4,6-Dihydroxy-2-methylpyrimidine is a reagent used in chemical synthesis.

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 17 fragments, suitable for gel electrophoresis. The DNA includes fragments ranging from 50-1,500 base pairs. The 200 and 500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 g a load) for approximating the mass of DNA in comparably intense samples of a similar size.The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

6-Dimethylamino-9-(b-D-ribofuranosyl)purine is a methyladenosine compound functioning as a heterocyclic building block and reagent in nucleotide chemistry. It’s composed of two methyl groups bound to an adenine nucleobase. 6-Dimethylamino-9-(b-D-ribofuranosyl)purine has roles in nucleic composition. Modified with fatty acids, the molecule has been shown to be effective against tumor activity in cultured L1210 leukemia and Ehrlich ascites.

The tertiary amino compound is a minor rRNA nucleoside responsible for structure. Antibodies to 6-Dimethylamino-9-(b-D-ribofuranosyl)purine located the nucleoside in bacterial ribosomes, placing it at the 30S subunit of E. coli near the 3’ end; it is not present in kasugamycon-resistant mutants. In peas, chromatographic tests confirmed conservation in chloroplast rRNA subunits analogous to those in E. coli. Residues of this substance were likewise present in wheat germ at the small 18S and 40S RNA subunit particles. It’s been located in the tRNA of Mycobacterium bovis Bacille Calmette-Guérin (BCG) as well.

GoldBio's 6x Blue DNA Loading Dye is pre-mixed buffer designed for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 4,000 bp and 500 bp on a standard 1% TAE agarose gel respectively (800 bp and 150 bp on a 2% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

GoldBio's 6x Green DNA Loading Dye is a pre-mixed buffer for tracking DNA samples during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 4,000 bp and 50 bp on a standard 1% TAE agarose gel respectively (800 bp and 30 bp on a 2% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21 (DE3)
Species: E. coli
Transformation efficiency: ≥5 x 10⁵ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s BL21 (DE3) Electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature ≥1 x 10⁸ cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1(fhuA2) and are B strain.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 10⁸ cfu/µg pUC19 DNA
Blue/white screening: Yes

Arginine is an essential amino acid found in food and plant sources, and many biological systems. D-Arginine specifically can inhibit arginine decarboxylase enzymes and also functions as a metabolite.

D-Cysteine is the enantiomer of L-Cysteine and is not typically found in natural systems. D-Cysteine is typically used as a reagent for more complicated biological molecules like N-acetyl derivatives for inhibiting cell apoptosis. It can also be used as a metabolite that is not readily converted by enzymes like its more common enantiomer L-Cysteine.

D-Galactose is a monosaccharide sugar typically abbreviated Gal. This specific form of D-Galactose is derived from peach gum which is important when making recombinant proteins for therapeutic purposes in order to avoid animal diseases like Bovine Spongiform Encephalopathy.

D-Glucose 6-phosphate barium salt, derived from adding a barium molecule to the compound D-(+)-Glucose, is a monosaccharide derivative and organic phosphate. This carbohydrate appears as a white powder and can be applied as a chiral reagent. Its tendency for separation has contributed to research in circulatory systems and treating liquids with solid sorbents. In hydrogel analysis, it showed consistent binding affinity to d-glucose, l-glucose, d-fructose and d-gluconamide as other similar polymers; it was not selective for either glucose enantiomer.

D-Glucose 6-phosphate is an important phosphorylated sugar found in the cell, and a majority of the glucose entering a cell is of this chemical conformation. It forms the start of two different metabolic pathways; glycolysis and the pentose phosphate pathway, or it may find itself converted into glycogen or starch for storage. It is the main substrate for glucose-6-phosphatase enzyme. It can be used as an energy source for Myobacterium tuberculosis and inhibits activity of sarcoendoplasmic reticulum calcium ATPase in rat brains, lowering calcium accumulation in the endoplasmic reticulum. When reacted to form 2-deoxy-D-glucose-6-phosphate it can be used to inhibit bovine heart hexokinase.

Leucine is an alpha-amino acid used in the biosynthesis of proteins that is not synthesized by animals and must be ingested. Leucine is an activator of mTOR, stimulating muscle protein synthesis. Leucine is also used in hemoglobin formation.

D-Leucine has been used to protect mice from seizures and D-leucine specifically terminates seizures in mice after in the onset of seizure activity, while in breast cancers it can inhibit system L(LAT1) based growth. D-Leucine also triggers the disassembly of amyloid fibers linking biofilms.

Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.

Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).

Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.

Note: The Free Acid form of luciferin will not dissolve in water or PBS unless a dilute base such as NaOH, KOH, sodium bicarbonate or potassium bicarbonate is added to adjust the pH. The potassium or sodium salts of luciferin are more convenient for experiments, particularly for in vivo imaging, since they readily dissolve in water or buffer.

Tested and Certified for in vivo imaging (See "Luciferin FAQ" in additional information)

Firefly Luciferin is identical to Beetle Luciferin. (See "Additional Information" for structures.)

Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg²⁺ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.

Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).

Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.

Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.

Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).

Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays. ^®