Crosslinked Agarose Beads
4% Agarose Beads, Standard, Crosslinked
Catalog ID | Size | Pricing | |
---|---|---|---|
A-192-500 | 500 mL | $ 689.00 | |
A-192-1000 | 1 L | $ 1,034.00 |
Description
Agarose is a very inert polysaccharide that forms hydrophilic and high gel strength gels at low concentrations.
Agarose beads are microspheres of agarose gels with different particle diameters and concentrations. Small spherical particles of agarose act as a porous gel to filter or separate a mixture of molecules according to their individual sizes. Due to its chemical structure (easy to activate), the agarose beads may be prepared to bind biomolecules in a reversible or irreversible manner.
Cross-linked agarose beads are used in gel filtration chromatography (or molecular exclusion chromatography) as well as for activating beads for biomolecule purification or immobilization.
Cross-linked agarose beads can also be used for activating processes, generating active groups inside its pores capable of reversible or irreversible biomolecule bonds. Due to the bead’s large internal surface and to its composition (inert polysaccharide), agarose is an ideal medium for the preparation of activated beads.
Product Specifications
Catalog ID | A-192 |
---|---|
Name(s) | 4% Agarose Beads, Standard, Crosslinked |
Bead Size | Spherical, Standard: ~50-150 μm (approx.) |
Cross-linking | Yes |
Agarose % | 4% agarose |
Chemical Stability | Stable in all commonly used reagents. |
Storage Buffer | 20% Ethanol |
Storage/Handling | Store at 4°C. Do NOT freeze. |
4% HTC Agarose Beads, Standard
Catalog ID | Size | Pricing | |
---|---|---|---|
R-200-500 | 500 mL | $ 1,004.00 | |
R-200-1000 | 1 L | $ 1,421.00 |
Description
This matrix is a highly cross-linked 4% agarose bead with a bead size of 50-150 µm. It is a gel filtration resin developed for industrial-scale separation of large molecules and virus particles utilizing higher pressure and flow rates rather than low pressure standard beads.
This matrix is not preactivated. For preactivated resins, look for Glyoxal Agarose Beads or Protein G.
Product Specifications
Catalog ID | R-200 |
---|---|
Name(s) | 4% HTC Agarose Beads, Standard |
Bead Size | Spherical, Standard: ~50-150 μm (approx.) |
Cross-linking | Highly crosslinked agarose beads |
Temperature Stability | Autoclavable at 120°C for 20 min in H2O |
pH Stability |
Short term - pH 1.8-14 Long term - pH 3.8-13 |
Chemical Stability | Most commonly used aqueous and organic solutions including: 1 M NaOH, 8M Urea, 5M guanidine HCl, or 75% ethanol |
Exclusion Limit | ~3 x 107 |
Maximum Flow Rate | ≥500 cm/h at 15 cm bed height |
Maxium Pressure | ≥150 kPa at 15 cm bed height |
Storage Buffer | 20% EtOH |
Storage/Handling | Store at 4°C. Do NOT freeze. |
6% Agarose Beads, Standard, Crosslinked
Catalog ID | Size | Pricing | |
---|---|---|---|
A-193-500 | 500 mL | $ 709.00 | |
A-193-1000 | 1 L | $ 1,053.00 |
Description
Agarose is a very inert polysaccharide that forms hydrophilic and high gel strength gels at low concentrations.
Agarose beads are microspheres of agarose gels with different particle diameters and concentrations. Small spherical particles of agarose act as a porous gel to filter or separate a mixture of molecules according to their individual sizes. Due to its chemical structure (easy to activate), the agarose beads may be prepared to bind biomolecules in a reversible or irreversible manner. Cross-linked agarose beads are used in gel filtration chromatography (or molecular exclusion chromatography) as well as for activating beads for biomolecule purification or immobilization.
Cross-linked agarose beads can also be used for activating processes, generating active groups inside its pores capable of reversible or irreversible biomolecule bonds. Due to the bead’s large internal surface and to its composition (inert polysaccharide), agarose is an ideal medium for the preparation of activated beads.
Product Specifications
Catalog ID | A-193 |
---|---|
Name(s) | 6% Agarose Beads, Standard, Crosslinked |
Bead Size | Spherical, Standard: ~50-150 μm (approx.) |
Cross-linking | Yes |
Agarose % | 6% agarose |
Chemical Stability | Stable in all commonly used reagents. |
Storage Buffer | 20% Ethanol |
Storage/Handling | Store at 4°C. Do NOT freeze. |
6% HTC Agarose Beads, Standard
Catalog ID | Size | Pricing | |
---|---|---|---|
R-201-500 | 500 mL | $ 1,090.00 | |
R-201-1000 | 1 L | $ 1,482.00 |
Description
This matrix is a highly crosslinked 6% agarose bead with a bead size of 50-150 µm. It is a gel filtration resin developed for industrial-scale separation of large molecules and virus particles utilizing higher pressure and flow rates rather than low pressure Standard Beads.
This matrix is not preactivated. For preactivated resins, look for Glyoxal Agarose Beads or Protein G.
Product Specifications
Catalog ID | R-201 |
---|---|
Name(s) | 6% HTC Agarose Beads, Standard |
Bead Size | Spherical, Standard: ~50-150 μm (approx.) |
Cross-linking | Highly crosslinked agarose beads |
Temperature Stability | Autoclavable at 120°C for 20 min in H2O |
pH Stability |
Short term - pH 1.8-14 Long term - pH 3.8-13 |
Chemical Stability | Most commonly used aqueous and organic solutions including: 1 M NaOH, 8M Urea, 5M guanidine HCl, or 75% ethanol |
Exclusion Limit | ~4 x 106 |
Maximum Flow Rate | ≥1000 cm/h at 15 cm bed height |
Maxium Pressure | ≥300 kPa at 15 cm bed height |
Storage Buffer | 20% EtOH |
Storage/Handling | Store at 4°C. Do NOT freeze. |
Cobalt HTC Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
R-203-25 | 25 mL | $ 315.00 | |
R-203-100 | 100 mL | $ 794.00 | |
R-203-500 | 500 mL | $ 3,053.00 |
Description
Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).
Product Specifications
Catalog ID | R-203 |
---|---|
Name(s) | Cobalt HTC Agarose Beads |
Bead Size | Spherical, Standard: ~50-150 μm (approx.) |
Cross-linking | Highly crosslinked |
Agarose % | 6% agarose |
Binding/Loading Capacity | ~15 (μmol Me2+/ml gel) |
Maximum Flow Rate | 1000 cm/h at 15 cm bed height |
Maxium Pressure | 300 kPa at 15 cm bed height |
Chemical Stability | Stable in all commonly used reagents. |
Temperature Stability | Autoclavable at 120°C for 20 min in H2O |
Storage Buffer | 20% Ethanol |
Storage/Handling | Store at 4°C. Do NOT freeze. |
Glyoxal HTC 6% HIGH Density Agarose Beads

Catalog ID | Size | Pricing | |
---|---|---|---|
G-312-25 | 25 mL | $ 224.00 | |
G-312-100 | 100 mL | $ 674.00 |
Description
Glyoxal HTC 6% HIGH Density Agarose beads allow covalent binding of agarose to lysine amino groups (similar orientation to CNBr).
This product is adequate to work in large scale process (High Pressure). The resin has a high amount of aldehyde groups (40-60 µmoles Glyoxal / ml gel) providing multiple binding points and high immobilized ligand stability. The resin has been manufactured using 6% HTC agarose beads, and the pore size is adequate to work with enzymes, proteins & small ligands. The stability at high flow is adequate for large scale process.
Product Specifications
Catalog ID | G-312 |
---|---|
Name(s) | Glyoxal HTC 6% HIGH Density Agarose Beads |
Bead Size | Spherical, Standard: ~50-150 μm (approx.) |
Cross-linking | Highly crosslinked |
Activity | 40-60 (μmol glyoxyl/ml gel) |
Agarose % | 6% agarose |
Chemical Stability | Stable in all commonly used reagents. |
Temperature Stability | Autoclavable at 120°C for 20 min in H2O |
Exclusion Limit | >4x106 |
Storage Buffer | 20% Ethanol |
Storage/Handling | Store at 4°C. Do NOT freeze. |
Highest Density Nickel

Catalog ID | Size | Pricing | |
---|---|---|---|
H-390-25 | 25 mL | $ 360.00 | |
H-390-100 | 100 mL | $ 1,385.00 | |
H-390-500 | 500 mL | $ 6,217.00 |
Description
GoldBio’s new Highest Density Nickel Agarose is a high capacity resin manufactured with a polychelator ligand. The product was developed to work in the presence of EDTA and DTT without any loss of performance. Its specificity and stability allows a one-step purification, eliminating the need for pretreatment of samples that cause nickel stripping.
Our Highest Density Nickel Agarose is supplied as a suspension in 50% aqueous suspension containing 20% ethanol. Purifications can be performed using 20mM EDTA and 20mM DTT and can work in both native as well as denaturing conditions.
Nickel HTC Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
R-202-25 | 25 mL | $ 315.00 | |
R-202-100 | 100 mL | $ 794.00 | |
R-202-500 | 500 mL | $ 3,053.00 |
Description
Nickel HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Nickel purification with the high flow rates of the HTC resin (70% higher than commercial standards).
HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates, with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and Hydrophobic interaction chromatography resins.
Product Specifications
Catalog ID | R-202 |
---|---|
Name(s) | Nickel HTC Agarose Beads |
Bead Size | Spherical, Standard: ~50-150 μm (approx.) |
Cross-linking | Highly crosslinked |
Agarose % | 6% agarose |
Binding/Loading Capacity | ~20 (μmol Me2+/ml gel) |
Maximum Flow Rate | 1000 cm/h at 15 cm bed height |
Maxium Pressure | 300 kPa at 15 cm bed height |
Chemical Stability | Stable in all commonly used reagents. |
Temperature Stability | Autoclavable at 120°C for 20 min in H2O |
Storage Buffer | 20% Ethanol |
Storage/Handling | Store at 4°C. Do NOT freeze. |
Nickel NTA HTC Agarose Beads

Catalog ID | Size | Pricing | |
---|---|---|---|
H-355-25 | 25 mL | $ 334.00 | |
H-355-100 | 100 mL | $ 1,117.00 | |
H-355-500 | 500 mL | $ 4,801.00 |
Description
Nickel NTA HTC Agarose Resin has a binding capacity of ~60 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.
NTA cross-linked Agarose resin consists of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.
Product Specifications
Catalog ID | H-355 |
---|---|
Name(s) | Nickel NTA HTC Agarose Beads |
Bead Size | Spherical, Standard: ~50-150 μm (approx.) |
Cross-linking | Highly crosslinked |
Agarose % | 6% agarose |
Binding/Loading Capacity | ≥60 mg/ml gel |
Ligand | Nitrilotriacetic Acid (NTA) |
Storage Buffer | 20% Ethanol |
Storage/Handling | Store at 4°C. Do NOT freeze. |
Protein G HTC Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
P-430-5 | 5 mL | $ 386.00 | |
P-430-25 | 25 mL | $ 1,344.00 |
Description
Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains of native Protein G have been removed to ensure the maximum specific IgG binding capacity.
GoldBio offers rProtein G resins to isolate and purify classes, subclasses and fragments of immunoglobulins from cell culture media and biological fluids. Rapid purification and high yield of purified immunoglobulin are obtainable by this method.
Protein G is covalently bound to highly cross-linked agarose beads, providing a very stable bond that can greatly minimize leakage of the Protein G and allowing for reuse of the affinity resin in several purification steps. This resin works well in High Flow conditions.
Product Specifications
Catalog ID | P-430 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |