Cobalt
Cobalt agarose beads are used in His-tag purifications. GoldBio’s IDA cross-linked agarose resins consist of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. Cobalt divalent metal ions have a high specificity and low affinity binding capacity for the best purification of specific proteins from a protein extract. Cobalt has a binding capacity of 6-12 mg/ml with a ~30 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. GoldBio’s cobalt agarose beads are a high density form that helps to improve the affinity of the purification.
Showing 1-2 of 2 items.
Cobalt Agarose Beads (High Density)
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| H-310-5 | 5 mL | $ 59.00 | |
| H-310-10 | 10 mL | $ 99.00 | |
| H-310-25 | 25 mL | $ 219.00 | |
| H-310-50 | 50 mL | $ 365.00 | |
| H-310-100 | 100 mL | $ 649.00 | |
| H-310-500 | 500 mL | $ 2,645.00 |
Description
Available in bulk or in prepacked columns, cobalt has a binding capacity of >25 mg/ml with approximately 60 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. In addition, it allows for purification of proteins under native or denaturing conditions. Cobalt Agarose works very well with Ni/Cu/Co Resin Wash Buffer and Histidine Protein Elution Buffer.
Product Specifications
| Catalog ID | H-310 |
|---|---|
| Storage/Handling | store at 4°C. Do NOT freeze. |
Cobalt HTC Agarose Beads
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| R-203-25 | 25 mL | $ 306.00 | |
| R-203-100 | 100 mL | $ 772.00 | |
| R-203-500 | 500 mL | $ 2,965.00 |
Description
Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).
Product Specifications
| Catalog ID | R-203 |
|---|---|
| Storage/Handling | store at 4°C. Do NOT freeze. |
