Cell Lysis

The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Cellulase R-10, also called Cellulase "Onozuka" R-10, is derived from Trichoderma Viride. Cellulase R-10 is a multi-enzymatic system consisting of various enzymes, including cellulase, hemicellulase, pectinase and protease. Cellulase R-10 is typically used in association with macerozyme R-10 in the decomposition of plant cell walls to produce protoplasts.

Cellulase “Onozuka RS” is produced by a mutant Trichoderma viride that was derived from the parent strain for Cellulase “Onozuka -R-10”. Cellulase RS contains a very high activity of decomposing natural celluloses. This type of cellulase can be used to obtain protoplasts in a very short time and dissolves cell walls of a wider range of plants.

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and a phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca2+.

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca 2+.

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5 mM Ca²⁺.

Guanidine HCl is a freely soluble chaotropic agent routinely used for protein denaturation and folding.

Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between the N-acetylmuramic acid and the N-acetyl-D-glucosamine residues in peptidoglycan.

Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence of EDTA.

Macerozyme R-10 is a macerating enzyme from the Rhizopus sp. which is perfectly suited for the isolation of plant cells. Macerozyme R-10 is often used in combination with Cellulase "Onozuka R-10" in the isolation of plant protoplasts.

Mammalian Cell Lysis Buffer has been developed for extraction of total soluble proteins from mammalian cultured cells. The Mammalian Cell Lysis Buffer is based on organic buffering agents, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Mammalian Cell Lysis Buffer. Mammalian Cell Lysis Buffer reagent has been tested for use with a wide variety of mammalian cells. Mammalian Cell Lysis Buffer can be used for both suspension as well as adherent cells. The proprietary combination of this reagent provides a simple and versatile method for the extraction of proteins from mammalian cells.

Tissue Cell Lysis Buffer has been developed for extraction of total soluble protein from fresh or frozen animal tissues. Tissue Cell Lysis Buffer is based on an organic buffer, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Tissue Cell Lysis Buffer. Tissue Cell Lysis Buffer reagent has been tested for use with a wide variety of animal tissues.

The Yeast Lysis Buffer Kit is useful for extraction of soluble proteins from yeast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.

This kit is provided with an optional protocol to make spheroplast and remove lytic enzyme Zymolyase®, prior to lysis and extraction of yeast proteins. Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase® preparation is also provided. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer.

The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works with both gram-positive and gram-negative bacteria. The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast Lysis Buffer eliminates the need for laborious glass bead lysis of yeast cells.

This kit is suitable for processing 100 x 50 µl yeast cell pellet preparations. Yeast Lysis Buffer is also available separately for further downstream processing.

The Yeast Spheroplast Lysis Buffer is useful for extraction of soluble proteins from spheroplast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.

Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer. The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works well with both gram-positive and gram-negative bacteria.

The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast Lysis Buffer eliminates the need for laborious glass bead lysis of yeast cells.