Buffers & Washes for Chromatography
5X Phosphate Buffer + 3M Imidazole Solution
Catalog ID | Size | Pricing | |
---|---|---|---|
I-905-100 | 100 mL | $ 232.00 |
Description
This Buffer Set consists of:
- 5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl)
- 25 mL 3M Recrystallized Imidazole Solution, pH 6.0
This Buffer set allows researchers to develop, wash and elute buffers for His-tag protein purification.
Product Specifications
Catalog ID | I-905 |
---|
5X Phosphate Buffer, pH 8
Catalog ID | Size | Pricing | |
---|---|---|---|
P-500-50 | 50 mL | $ 82.00 | |
P-500-100 | 100 mL | $ 119.00 | |
P-500-250 | 250 mL | $ 160.00 |
Description
5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl).
Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4). This buffer is the basis for wash and elution buffers for protein purification through His-tag.
Product Specifications
Catalog ID | P-500 |
---|---|
Storage/Handling | Store at 4°C. |
His-Tag Buffer Set
Catalog ID | Size | Pricing | |
---|---|---|---|
I-906-250 | 1 kit | $ 144.00 |
Description
His-Tag Buffer Set includes:
- 250 mL Wash Buffer (50mM Phosphate Buffer pH 8, 300mM NaCl, 10mM Imidazole)
- 50 mL Elution Buffer (50mM Phosphate Buffer, 300mM NaCl, 500mM Imidazole)
Designed for use with Goldbio Nickel and Cobalt Agarose resins, but it can be used with most standard His-tag purification resins.
Product Specifications
Catalog ID | I-906 |
---|
Imidazole - 3M Recrystallized Solution, pH 6.0
Catalog ID | Size | Pricing | |
---|---|---|---|
I-901-25 | 25 mL | $ 92.00 | |
I-901-50 | 50 mL | $ 140.00 | |
I-901-100 | 100 mL | $ 212.00 | |
I-901-250 | 250 mL | $ 473.00 |
Description
Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.
Product Specifications
Catalog ID | I-901 |
---|---|
CAS # | 288-32-4 |
MW | 68.08 g/mol |
Storage/Handling | Store at 4°C. |
Imidazole, Recrystallized
Catalog ID | Size | Pricing | |
---|---|---|---|
I-902-25 | 25 g | $ 82.00 | |
I-902-50 | 50 g | $ 129.00 | |
I-902-100 | 100 g | $ 212.00 | |
I-902-500 | 500 g | $ 628.00 |
Description
Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.
Product Specifications
Catalog ID | I-902 |
---|---|
CAS # | 288-32-4 |
MW | 68.08 g/mol |
Storage/Handling | Store at room temperature. |
UN Number | 3263 |
Item Class | 8 |
Group Number | III |