5-Fluoroorotic Acid
5-Fluoroorotic Acid (5-FOA) is a widely used chemical in yeast genetics. When introduced into a media carrying a selectively modified yeast it will be convert into 5-fluorouracil, which kills off non-modified yeast strains. This can be used across a wide variety of yeast strains, like URA3, URA 4 and URA 5, and especially useful for mutant strains. GoldBio 5-FOA is an affordable way to control your yeast experiment.
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5-Fluoroorotic acid monohydrate (5-FOA)
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| F-230-2.5 | 2.5 g | $ 75.00 | |
| F-230-5 | 5 g | $ 135.00 | |
| F-230-10 | 10 g | $ 225.00 | |
| F-230-25 | 25 g | $ 409.00 |
Description
5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.
Product Specifications
| Catalog ID | F-230 |
|---|---|
| CAS # | 220141-70-8 |
| MW | 192.10 g/mol |
| Grade | MOLECULAR BIOLOGY GRADE |
| Storage/Handling | Store at -20°C. Protect from light. |
