Chances are that if your research involves affinity chromatography, then you’re probably already using or have previously used Cyanogen Bromide-activated (CNBr-activated) agarose beads to bind or purify your proteins, antibodies or enzymes. That’s the industry standard for ligand immobilization! It’s great! It works! It’s wonderful!
But what if Gold Bio could offer you something better...and less expensive?
CNBr is often used to immobilize proteins by coupling with agarose beads. Usually, the agarose is either 4% or 6% (which typically sets the exclusion limit of the beads) and can be crosslinked or not (crosslinking permanently binds the linker to the agarose which offers the advantage of autoclaving the beads and/or re-using them). CNBr is used so often because it reacts simply and easily with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates, which then react with the amino group of proteins, binding them to the agarose. But CNBr is quite toxic and it’s also very sensitive to oxidation. And its ligand binding is relatively unstable because of its isourea bond which tends to act as an anion exchanger.
At Gold Bio, we want to provide you with the best reagents possible for your research. To that end, we offer two alternatives to the classic CNBr resin: Glyoxal- and Aminoethyl-activated agarose beads!
Our Glyoxal/Aminoethyl agarose beads are all crosslinked, so that you can autoclave them and re-use them multiple times. They are covalently bound to the agarose, giving them a qualitative advantage to resins activated by CNBr. They have a higher binding capacity, faster conjugation, more reproducibility and a longer shelf life than CNBr. You can find a comparison study of Human IgG binding between Glyoxal and CNBr agarose in the Additional Information of the products in the Glyoxal section or Aminoethyl section of our website.
For another comparison of binding capacity, check out this chart
The combination of Glyoxal/Aminoethyl system offers more versatility for your research. While you would use the Glyoxal resin to bind with the amino group of your protein (similar to CNBr), you can use the Aminoethyl resin to bind with the carboxy group of your protein, an opportunity that CNBr resins cannot match!
You can find a detailed list of comparable Gold Bio products to some of the more common CNBr resins in the Additional Information of either the Glyoxal or Aminoethyl products. If you still have questions about these products or about which one you should try, please contact our tech support and we will be glad to help!
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