Auxo-Agro™ Agrobacterium Competent Cells
Are antibiotics hurting your plant transformants?
Agrobacterium removal is needed following a successful plant transformation. Traditionally, antibiotics are used for this purpose, but higher concentrations of antibiotics can have an adverse effect on plant tissue.
GoldBio EHA105∆Met1 and LBA4404∆Met1 Agrobacterium tumefaciens competent cells minimize the use of antibiotics to prevent overgrowth of the plant tissue and can help streamline your plant transformation procedures.
Benefits:
- Reduces the need for antibiotics during transformation.
- Increases plant transformation efficiency.
- Agrobacterium can be completely killed by removing methionine from the culture media combined with a low concentration of antibiotic.
- Knocking out genes to cause auxotrophy DOES NOT affect transformation capacity.1
- Prías-Blanco M, Chappell TM, Freed EF, Illa-Berenguer E, Eckert CA, Parrott WA. An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation. Transgenic Res. 202231: 661-676. doi: 10.1007/s11248-022-00328-4. PMID: 36239844.
Important note from this paper: “Switchgrass transformation was chosen to validate these strains by evaluating their performance in a difficult transformation system."
Figures: Methionine dependent growth of auxotrophic strains after 24-hour incubation at 28°C in minimal medium (M9) or M9 supplemented with DO (LBA4404 on left, EHA105 on right)1
Auxo-Agro™ LBA4404 Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-267-5x50 | 5 x 50 μL |
|
$ 164.00 |
| CC-267-10x50 | 10 x 50 μL |
|
$ 296.00 |
| CC-267-15x50 | 15 x 50 μL |
|
$ 400.00 |
Description
GoldBio’s new Auxo-Agro™ competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.
Our LBA4404 strain of Agrobacterium tumefaciens can be used in genetic transformation of tomato, tobacco and other plants After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain which can create a more difficult experimental protocol. Auxo-Agro™ cells help to solve this problem when dual selection is performed in both minimal media without Methionine in combination with selective antibiotics, such as Timentin, Cefotaxime or Meropenem.
GoldBio’s LBA4404 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octopine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: LBA4404∆Met
Format: Tubes
Transformation efficiency: ≥4 x 106 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Auxo-Agro™ EHA105 Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-268-5x50 | 5 x 50 μL |
|
$ 164.00 |
| CC-268-10x50 | 10 x 50 μL |
|
$ 296.00 |
| CC-268-15x50 | 15 x 50 μL |
|
$ 400.00 |
Description
GoldBio’s new Auxo-Agro™ competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.
Our EHA105 strain of Agrobacterium tumefaciens can be used in genetic transformation of tomato, tobacco and other plants. After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain which can create a more difficult experimental protocol. Auxo-Agro™ cells help to solve this problem when selection is performed in both minimal media without Methionine in combination with selective antibiotics, such as Timentin, Cefotaxime or Meropenem.
GoldBio’s EHA105 Agrobacterium electrocompetent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain harbors a rifampicin resistance (rif) gene.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105∆Met
Format: Tubes
Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Auxo-Agro™ LBA4404 Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-167-5x50 | 5 x 50 μL |
|
$ 156.00 |
| CC-167-10x50 | 10 x 50 μL |
|
$ 276.00 |
| CC-167-15x50 | 15 x 50 μL |
|
$ 370.00 |
Description
GoldBio’s new Auxo-Agro™ competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.
Our LBA4404 strain of Agrobacterium tumefaciens can be used in genetic transformation of tomato, tobacco and other plants. After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain which can create a more difficult experimental protocol. Auxo-Agro™ cells help to solve this problem when selection is performed in both minimal media without Methionine in combination with selective antibiotics, such as Timentin, Cefotaxime or Meropenem.
GoldBio’s LBA4404 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbors a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octopine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: LBA4404∆Met
Format: Tubes
Transformation efficiency: ≥1 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Auxo-Agro™ EHA105 Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-168-5x50 | 5 x 50 μL |
|
$ 156.00 |
| CC-168-10x50 | 10 x 50 μL |
|
$ 276.00 |
| CC-168-15x50 | 15 x 50 μL |
|
$ 370.00 |
Description
GoldBio’s new Auxo-Agro™ competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.
Our EHA105 strain of Agrobacterium tumefaciens
can be used in genetic transformation of tomato, tobacco and other
plants. After transformation, antibiotics are commonly used to remove
Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain
which can create a more difficult experimental protocol. Auxo-Agro™
cells help to solve this problem when selection is performed in both
minimal media without Methionine in combination with selective
antibiotics, such as Timentin, Cefotaxime or Meropenem.
GoldBio’s EHA105 Agrobacterium chemically competent cells allow you
to obtain high transformation efficiency in applications such as gDNA or
cDNA library construction. Our EHA105 strain harbors a rifampicin resistance (rif) gene.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105∆Met
Format: Tubes
Transformation efficiency: ≥1 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
