Auxo-Agro™ Agrobacterium Competent Cells

Are antibiotics hurting your plant transformants?

Agrobacterium removal is needed following a successful plant transformation. Traditionally, antibiotics are used for this purpose, but higher concentrations of antibiotics can have an adverse effect on plant tissue.

GoldBio EHA105∆Met¹ and LBA4404∆Met¹ Agrobacterium tumefaciens competent cells minimize the use of antibiotics to prevent overgrowth of the plant tissue and can help streamline your plant transformation procedures.

Benefits:

• Reduces the need for antibiotics during transformation.

• Increases plant transformation efficiency.

• Agrobacterium can be completely killed by removing methionine from the culture media combined with a low concentration of antibiotic.

• Knocking out genes to cause auxotrophy DOES NOT affect transformation capacity.¹

1. Prías-Blanco M, Chappell TM, Freed EF, Illa-Berenguer E, Eckert CA, Parrott WA. An Agrobacterium strain auxotrophic for methionine is useful for switchgrass transformation. Transgenic Res. 202231: 661-676. doi: 10.1007/s11248-022-00328-4. PMID: 36239844.
   Important note from this paper: “Switchgrass transformation was chosen to validate these strains by evaluating their performance in a difficult transformation system."

Figures: Methionine dependent growth of auxotrophic strains after 24-hour incubation at 28°C in minimal medium (M9) or M9 supplemented with DO (LBA4404 on left, EHA105 on right)¹