Articles

How to Regenerate Glutathione Resin

“You young people view everything as single-use and disposable.” My Ph.D. advisor had just found out...

How to Prepare Glutathione Elution Buffer

If you’re purifying GST or a GST-fusion protein with glutathione agarose beads, then a key step in t...

What’s the Binding Capacity of Glutathione Agarose

You want to purify Glutathione S-transferase (GST) or a GST-fusion protein. So, you pull your GoldBi...

What is Glutathione?

Glutathione is a tripeptide antioxidant produced by our bodies that plays a really important role in...

How Biotinylated Molecules are Purified with Streptavidin Beads

treptavidin is a bacterial protein that binds to the small molecule biotin with extremely high affin...

How to Elute Biotinylated Proteins and Nucleic Acids from Streptavidin Beads

Streptavidin is a bacterial protein that binds to the small molecule biotin with extremely tight aff...

What Does the DE3 Represent in Competent Cells Like BL21(DE3) and DL39(DE3)?*

Expression systems are important tools in molecular biology, enabling researchers to produce protein...

How Much Streptavidin Resin Should I use? Binding Capacity has the Answer

en using streptavidin agarose beads to purify biotinylated nucleic acids or proteins, you’ll need to...

How do I Get my Protein Out of Inclusion Bodies?

Have you ever had a problem where you had to try many different potential solutions before you final...