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How to Regenerate Glutathione Resin
“You young people view everything as single-use and disposable.” My Ph.D. advisor had just found out...

How to Prepare Glutathione Elution Buffer
If you’re purifying GST or a GST-fusion protein with glutathione agarose beads, then a key step in t...

What’s the Binding Capacity of Glutathione Agarose
You want to purify Glutathione S-transferase (GST) or a GST-fusion protein. So, you pull your GoldBi...

What is Glutathione?
Glutathione is a tripeptide antioxidant produced by our bodies that plays a really important role in...
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How Biotinylated Molecules are Purified with Streptavidin Beads
treptavidin is a bacterial protein that binds to the small molecule biotin with extremely high affin...

How to Elute Biotinylated Proteins and Nucleic Acids from Streptavidin Beads
Streptavidin is a bacterial protein that binds to the small molecule biotin with extremely tight aff...
What Does the DE3 Represent in Competent Cells Like BL21(DE3) and DL39(DE3)?*
Expression systems are important tools in molecular biology, enabling researchers to produce protein...

How Much Streptavidin Resin Should I use? Binding Capacity has the Answer
en using streptavidin agarose beads to purify biotinylated nucleic acids or proteins, you’ll need to...

How do I Get my Protein Out of Inclusion Bodies?
Have you ever had a problem where you had to try many different potential solutions before you final...