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How to Choose the Best Protein Purification Technique
If you need to purify a protein for your research, but you’re not sure exactly which purification te...

3 Common Reasons for Protein Isoelectric Point Discrepancies
Is your protein not sticking to the ion exchange column when you thought it should have? As frustrat...

3 Solubility Tags for Protein Affinity Purification
Let’s imagine that you are trying to express and purify a new protein. You attached a his-tag onto y...

Agarose Gel FAQs
These are some of the top questions surrounding gel electrophoresis and agarose gels. Learn about ho...

GST-Tagged Protein Purification
There are a number of great methods in the researcher’s toolbox for protein purification. Along wit...

His-Tag Metal Affinity Cations: What’s the difference again?
Unless you’ve done the equivalent of a post-doc on his-tag protein purification, you have probably a...

Troubleshooting: Purification of a Tagged Protein
Purification of a tagged protein may seem like a pretty routine task. You express your protein of in...