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3 Solubility Tags for Protein Affinity Purification
Let’s imagine that you are trying to express and purify a new protein. You attached a his-tag onto y...
Simple Overview of the 4 Common Techniques for Protein Purification
Proteins are the verbs of the cellular world. They perform important functions and carry out actions...
The 4 Types of Nucleic Acids Transfected Into Eukaryotic Cells
Choosing which type of nucleic acid you’d want to transfect depends on what you want to do in your e...
4 experimental Applications of Transfection
perimental applications using transfection include gene editing, modifying host cell gene expression...
3 Transfection Methods - A Basic Overview
There are three common transfection methods: chemical, physical and viral. Physical transfection use...
From Labs to Life: The Versatility of Purified Proteins in Practical Settings
Purified proteins help feed the world, keep us healthy, improve common products, and even begin solv...
E. coli Homologous Recombination: How It Works and Influences on Cloning
Homologous recombination is the exchange of sequences that occurs between two similar (homologous)...
Understanding Auxotrophic Mutants: Yeast and Bacterial Cloning Tools
Auxotrophic mutants are bacteria, yeast, protoplast or mammalian host cell strains that can’t produc...
Chelating Agents: A Beginner’s Introduction
A chelating agent is a compound that forms stable complexes with metal ions through different types...