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How do I Get my Protein Out of Inclusion Bodies?

Have you ever had a problem where you had to try many different potential solutions before you final...

Understanding Salting In and Salting Out: Salt as a Protein Purification Tool

Salt is a major component in protein purification buffers, and optimizing this important parameter c...

How to Use Magnetic Agarose Beads to Purify Antibodies: A Step-by-Step Protocol

This article provides a step-by-step protocol for purifying antibodies using Protein A, Protein G, o...

If you need to purify a protein for your research, but you’re not sure exactly which purification te...

Protein A is a bacterial protein from the cell wall of Staphylococus aureus that binds to the heavy...

Have you ever heard the phrase “measure twice, cut once?” In woodworking, this means making sure you...

Have you heard of DNA plasmids, sometimes called DNA vectors, but you aren’t quite sure what exactly...

AEBSF and PMSF are both irreversible protease inhibitors of serine proteases. With them both inhibit...

Ion Exchange Chromatography is routinely sandwiched between affinity and size exclusion steps in an...

Does it ever feel like the hardest part of a project is just getting started on the dang thing? For...

Have you ever heard the saying “opposites attract”? Well, it turns out this phrase is true in the mo...

Detergents are versatile chemical tools. But how exactly do they help in experiments? Some detergen...