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How do I Get my Protein Out of Inclusion Bodies?

Have you ever had a problem where you had to try many different potential solutions before you final...

Understanding Salting In and Salting Out: Salt as a Protein Purification Tool

Salt is a major component in protein purification buffers, and optimizing this important parameter c...

How to Use Magnetic Agarose Beads to Purify Antibodies: A Step-by-Step Protocol

This article provides a step-by-step protocol for purifying antibodies using Protein A, Protein G, o...

Selective media helps researchers identify their desired mutant host cell colonies during gene cloni...

Fluorescence microscopy spans from basic epifluorescence/widefield microscopy to advanced techniques...

There are seven different indications of problems with SDS-PAGE gel running. Some of these include s...

There are five different signs that indicate that there are problems with SDS-PAGE sample preparatio...

There are three signs that indicate issues with SDS-PAGE gel casting. These include bands not being...

What do you do with millions of data? It is a recurrent question in sequencing projects. Analyzing N...

Western blot, also called immunoblot, is a commonly used technique for qualitatively and quantitativ...

Proteins are determinants of cell, tissue and organ structure and physiology. Proteins are a class o...