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All About Affinity Purification of Antibodies
Antibodies are amazing! Antibodies from our immune cells help keep us healthy and mount a defense wh...

3 Solubility Tags for Protein Affinity Purification
Let’s imagine that you are trying to express and purify a new protein. You attached a his-tag onto y...

3 Small Peptide Tags for Affinity Protein Purification
Affinity purification is a frequently used technique to isolate proteins for further research and ot...

Buffer Preparation: Tips and Tricks
You’re preparing buffers in the lab, and maybe you’ve been following a protocol for the longest time...

4 experimental Applications of Transfection
perimental applications using transfection include gene editing, modifying host cell gene expression...

3 Transfection Methods - A Basic Overview
There are three common transfection methods: chemical, physical and viral. Physical transfection use...

What are E. coli Expression Strains?
E. coli expression strains, BL21(DE3) and DL39 for example, are used for recombinant RNA/protein exp...

Explain Alpha-Complementation and Blue-White Screening To Me Like I’m 10
Alpha-complementation – it’s a big phrase, but it’s not terribly hard to understand. The technical...

Selective Media Used in Gene Cloning
Selective media helps researchers identify their desired mutant host cell colonies during gene cloni...