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The 2 Common Types of Ion Exchange Chromatography: Anion vs. Cation
Ion exchange chromatography includes a suite of related methods that purify proteins based on their...
12 Useful Detergents for Your Experiments
You might be designing a cell lysis experiment to extract your target protein in its native form, or...
3 Common Reasons for Protein Isoelectric Point Discrepancies
Is your protein not sticking to the ion exchange column when you thought it should have? As frustrat...
Charged up: The Complexities and Significance of Protein Charge in Protein Purification
Do you know what really charges me up? Discussing protein electrostatics, of course! Proteins are m...
All About Affinity Purification of Antibodies
Antibodies are amazing! Antibodies from our immune cells help keep us healthy and mount a defense wh...
3 Solubility Tags for Protein Affinity Purification
Let’s imagine that you are trying to express and purify a new protein. You attached a his-tag onto y...
3 Small Peptide Tags for Affinity Protein Purification
Affinity purification is a frequently used technique to isolate proteins for further research and ot...
Buffer Preparation: Tips and Tricks
You’re preparing buffers in the lab, and maybe you’ve been following a protocol for the longest time...
4 experimental Applications of Transfection
perimental applications using transfection include gene editing, modifying host cell gene expression...