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How do I Get my Protein Out of Inclusion Bodies?
Have you ever had a problem where you had to try many different potential solutions before you final...
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Understanding Salting In and Salting Out: Salt as a Protein Purification Tool
Salt is a major component in protein purification buffers, and optimizing this important parameter c...
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How to Use Magnetic Agarose Beads to Purify Antibodies: A Step-by-Step Protocol
This article provides a step-by-step protocol for purifying antibodies using Protein A, Protein G, o...
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Answers, Tips and How-Tos

Maybe Dracula had it right? - Growth Factor in blood of young mice responsible for heart tissue rejuvenation in older mice.
In a remarkable paper published in the May 9th edition of Cell, two researchers at the Harvard Stem...

Antibody Purification with Protein A, G
To coincide with the introduction of our new Protein G antibody purification resin, we thought that...

Blue-White Screening
Today we’re going to shine a spotlight on a widely used technique in the field of molecular biology,...

Reducing Agents (Part 4 of 4) - TCEP-HCl (Tris-(carboxyethyl) phosphine hydrochloride)
Previously, we’ve been discussing the function of various reducing agents. There are a number of red...

Troubleshooting: Protein Expression
Last time on Troubleshooting, we covered Tagged Protein Purification, but what happens if your prote...

Troubleshooting: Purification of a Tagged Protein
Purification of a tagged protein may seem like a pretty routine task. You express your protein of in...