E. coli Cloning Strains for Molecular Biology: What They Are and Why They Matter
by Pallabi Roy Chakravarty, Ph.D.
The final concentration of IPTG used for induction varies from 0.1 to 1.0 mM, with 0.5 or 1.0 mM most frequently used. For proteins with...
A His-tag is a stretch of 6-10 histidine amino acids in a row that is used for affinity purification, protein detection, and biochemical assays. His-tags...
Competent cells such as DH5a, DH10B, and BL21 will maintain their transformation efficiency for at least a year with proper storage. It is important to...
Ni2+ ions give nickel agarose beads their characteristic blue color. This blue color can fade or disappear completely when loading his-tagged proteins onto the column....