Animal Free Recovery Medium
GoldBio’s competent cell recovery medium is now animal-free!
Recovery medium is a commonly used reagent for use in transformation with both Agrobacterium or E. coli cells. One of the primary ingredients of recovery media is a peptone called tryptone. Plant-based tryptone works just as well in recovery media and eliminate the safety, ethical, and environmental shortcomings of animal-based tryptone.
Peptones are protein mixtures that have been hydrolyzed, or cut into smaller peptides and amino acids, by an enzyme or by acid. These chopped up proteins serve as a source of nitrogen atoms and amino acids to supercharge cell growth. This support is especially needed in competent cell recovery medium when cells are recovering from stressful transformation processes.
Traditionally, peptones are often sourced from animal origins – such as meat or organs. However, there has been a transition away from animal-based peptides because of a variety of concerns.
For instance, animal-based peptones carry the risk of containing Transmissible Spongiform Encephalopathies (TSE) such as mad cow disease, foot and mouth disease, and even viral infections such as swine flu. Meaning that animal-based peptones can transmit these diseases when they are present. Thankfully, plant-based peptones do not transmit any of these safety risks.
Additionally, many consider plant-based peptones to be more ethical as it eliminates animals being conceived and raised just to harvest them for scientific reagents.
Lastly, plant-based peptones are better for the environment. Whereas animals release carbon dioxide (CO2) emissions into the atmosphere, plants ingest CO2 and convert it into the oxygen that we all breathe while they are growing.
Importantly, plant-based peptones work just as well, and sometimes better, than their animal-based counterparts, depending on the particular application. So, when you are using GoldBio’s Competent Cell Recovery Medium, you know that with our animal-based tryptone, our recovery media works just as well as animal-based formulations, and is safer for you, more ethical for animals, and better for the planet – now that’s a win-win-win-win scenario!
GoldBio Animal-Free products will be clearly labeled on the bottle beneath the name.
Animal-based peptones carry the risk of containing Transmissible Spongiform Encephalopathies (TSE) such as mad cow disease, foot and mouth disease, and even viral infections such as swine flu. Meaning that animal-based peptones can transmit these diseases when they are present. Thankfully, plant-based peptones do not transmit any of these safety risks.
Additionally, many consider plant-based peptones to be more ethical as it eliminates animals being conceived and raised just to harvest them for scientific reagents.
Lastly, plant-based peptones are better for the environment. Whereas animals release carbon dioxide (CO2) emissions into the atmosphere, plants ingest CO2 and convert it into the oxygen that we all breathe while they are growing.
These products are safer for the environment, do not require the destruction of animals for research, and are easier to import with little to no regulatory restrictions
No! Our animal-free media will work with your protocols and experiments with no changes required.
Agrobacterium Competent Cell Recovery Medium
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-301-A | 6 x 12 mL | $ 83.00 |
Description
GoldBio’s Agrobacterium Competent Cell Recovery Medium is an animal-free recovery medium specifically formulated for optimal competent cell transformations for Agrobacterium competent cells.
E. coli Competent Cell Recovery Medium
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-302-A | 6 x 12 mL | $ 83.00 |
Description
GoldBio’s E. Coli Competent Cell Recovery Medium is an animal-free recovery medium specifically formulated for optimal competent cell transformations for all E. coli competent cells.
GoldBio now carries a selection of our popular, high-quality competent cells made free of animal products, with the goal of all cells being completely animal-free. Check out the current list below of available competent cells made free of animal-derived products.
GB5-alpha™ Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-101-5x50 | 5 x 50 μL |
|
$ 48.00 |
| CC-101-5x100 | 5 x 100 μL |
|
$ 87.00 |
| CC-101-10x50 | 10 x 50 μL |
|
$ 87.00 |
| CC-101-15x50 | 15 x 50 μL |
|
$ 115.00 |
| CC-101-20x50 | 20 x 50 μL |
|
$ 136.00 |
| CC-101-10x100 | 10 x 100 μL |
|
$ 148.00 |
| CC-101-15x100 | 15 x 100 μL |
|
$ 200.00 |
Description
GoldBio’s GB5-alpha™ Chemically Competent E. coli cells are equivalent to DH5-alpha cells and are high efficiency cells suitable for transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.
Product Specifications
Competent cell type: Chemically Competent
Equivalent to: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes
Product Specifications
| Catalog ID | CC-101 |
|---|---|
| Name(s) | GB5-alpha™ Chemically Competent E. coli cells |
| Competent cell type | Chemically Competent |
| Species | E. coli |
| Transformation efficiency | ≥5 x 106 CFU/µg |
| Blue/white screening | Yes |
| Storage/Handling | Store Competent Cells at -80°C. |
BL21 Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-102-5x50 | 5 x 50 μL |
|
$ 48.00 |
| CC-102-10x50 | 10 x 50 μL |
|
$ 87.00 |
| CC-102-15x50 | 15 x 50 μL |
|
$ 115.00 |
Description
GoldBio’s BL21 Chemically Competent E. coli cells are a good, universal strain of cell that is gives great transformation for protein expression and induction, and is especially useful for non-T7 vectors protein expression systems. BL21 chemically competent cells feature a widely used host background, and are deficient in both lon (1) and ompT proteases. In addition, BL21 Chemically Competent E. coli cells are resistant to phage T1 (fhuA2).
Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes
Product Specifications
| Catalog ID | CC-102 |
|---|---|
| Name(s) | BL21 Chemically Competent E. coli Cells |
| Competent cell type | Chemically competent |
| Species | E. coli |
| Transformation efficiency | ≥1 x 106 CFU/µg |
| Blue/white screening | Yes |
| Storage/Handling | Store Competent Cells at -80°C. |
BL21 (DE3) Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-103-5x50 | 5 x 50 μL |
|
$ 48.00 |
| CC-103-10x50 | 10 x 50 μL |
|
$ 87.00 |
| CC-103-15x50 | 15 x 50 μL |
|
$ 115.00 |
| CC-103-20x50 | 20 x 50 μL |
|
$ 136.00 |
Description
GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.
Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21 (DE3)
Species: E. coli
Transformation efficiency: ≥5 x 105 cfu/µg pUC19 DNA
Blue/white screening: Yes
Product Specifications
| Catalog ID | CC-103 |
|---|---|
| Name(s) | BL21 (DE3) Chemically Competent E. coli Cells |
| Competent cell type | Chemically competent |
| Species | E. coli |
| Transformation efficiency | ≥5 x 105 CFU/µg |
| Blue/white screening | Yes |
| Storage/Handling | Store Competent Cells at -80°C. |
GV3101 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-105-5x50 | 5 x 50 μL |
|
$ 156.00 |
| CC-105-10x50 | 10 x 50 μL |
|
$ 276.00 |
| CC-105-15x50 | 15 x 50 μL |
|
$ 370.00 |
Description
GoldBio’s GV3101 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted in the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101
Transformation efficiency: ≥6 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Product Specifications
| Catalog ID | CC-105 |
|---|---|
| Name(s) | GV3101 Agrobacterium Chemically Competent Cells |
| Competent cell type | Chemically Competent |
| Species | A. tumefaciens |
| Transformation efficiency | ≥6 x 104 CFU/µg |
| Blue/white screening | No |
| Storage/Handling | Store Competent Cells at -80°C. |
AGL-1 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-106-5x50 | 5 x 50 μL |
|
$ 156.00 |
| CC-106-10x50 | 10 x 50 μL |
|
$ 276.00 |
| CC-106-15x50 | 15 x 50 μL |
|
$ 370.00 |
Description
GoldBio’s AGL-1 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥4 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Product Specifications
| Catalog ID | CC-106 |
|---|---|
| Name(s) | AGL-1 Agrobacterium Chemically Competent Cells |
| Competent cell type | Chemically Competent |
| Species | A. tumefaciens |
| Transformation efficiency | ≥4 x 104 CFU/µg |
| Blue/white screening | No |
| Storage/Handling | Store Competent Cells at -80°C. |
LBA4404 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-107-5x50 | 5 x 50 μL |
|
$ 156.00 |
| CC-107-10x50 | 10 x 50 μL |
|
$ 276.00 |
| CC-107-15x50 | 15 x 50 μL |
|
$ 370.00 |
Description
GoldBio’s LBA4404 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbors a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: LBA4404
Transformation efficiency: ≥1 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Product Specifications
| Catalog ID | CC-107 |
|---|---|
| Name(s) | LBA4404 Agrobacterium Chemically Competent Cells |
| Competent cell type | Chemically Competent |
| Species | A. tumefaciens |
| Transformation efficiency | ≥1 x 104 CFU/µg |
| Blue/white screening | No |
| Storage/Handling | Store Competent Cells at -80°C. |
LBA4404 Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-220-5x50 | 5 x 50 μL |
|
$ 164.00 |
| CC-220-10x50 | 10 x 50 μL |
|
$ 296.00 |
| CC-220-15x50 | 15 x 50 μL |
|
$ 400.00 |
Description
GoldBio’s LBA4404 Electrocompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: LBA4404
Transformation efficiency: ≥4 x 106 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Product Specifications
| Catalog ID | CC-220 |
|---|---|
| Name(s) | LBA4404 Agrobacterium Electrocompetent Cells |
| Competent cell type | Electrocompetent |
| Species | A. tumefaciens |
| Transformation efficiency | ≥4 x 106 CFU/µg |
| Blue/white screening | No |
| Storage/Handling | Store Competent Cells at -80°C. |
EHA105 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-108-5x50 | 5 x 50 μL |
|
$ 156.00 |
| CC-108-10x50 | 10 x 50 μL |
|
$ 276.00 |
| CC-108-15x50 | 15 x 50 μL |
|
$ 370.00 |
Description
GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥5 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Product Specifications
| Catalog ID | CC-108 |
|---|---|
| Name(s) | EHA105 Agrobacterium Chemically Competent Cells |
| Competent cell type | Chemically Competent |
| Species | A. tumefaciens |
| Transformation efficiency | ≥5 x 104 CFU/µg |
| Blue/white screening | No |
| Storage/Handling | Store Competent Cells at -80°C. |
RR1 Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-113-5x50 | 5 x 50 µl |
|
$ 48.00 |
| CC-113-10x50 | 10 x 50 µl |
|
$ 87.00 |
Description
GoldBio’s RR1 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli RR1 is a recA+ derivative of the HB101 strain and can be more useful than HB101 if a recA+ background is required.
Product Specifications
Competent cell type: Chemically competent
Derivative of: RR1
Species: E. coli
Format: Tubes
Transformation efficiency: ≥3 x 107 cfu/µg pUC19 DNA
Blue/white screening: No
Product Specifications
| Catalog ID | CC-113 |
|---|---|
| Name(s) | RR1 Chemically Competent E. coli Cells |
| Competent cell type | Chemically competent |
| Species | E. coli |
| Transformation efficiency | ≥3 x 107 CFU/µg |
| Blue/white screening | No |
| Storage/Handling | Store Competent Cells at -80°C. |
CJ236 Chemically Competent E. Coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-151-5x50 | 5 x 50 μL |
|
$ 47.00 |
| CC-151-10x50 | 10 x 50 μL |
|
$ 84.00 |
| CC-151-15x50 | 15 x 50 μL |
|
$ 111.00 |
Description
GoldBio CJ236 chemically competent E. coli cells are ideally suited for use in the Kunkel method of mutagenesis. CJ236 cells have part of their Thymine replaced with deoxyuracil in the DNA due to dut- and ung- mutations which prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. CJ236 contains the F’ gene which confers Chloramphenicol resistance.
CJ236 chemically competent E. coli cells are free of animal-derived products and grown with animal-free media.
Kit Components
- Competent Cells
- 1 x 12 mL Recovery Media
- 1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL)
Product Specifications
| Catalog ID | CC-151 |
|---|---|
| Name(s) | CJ236 Chemically Competent E. Coli Cells |
| Competent cell type | Chemically competent |
| Species | E. Coli |
| Transformation efficiency | ≥1 x 104 CFU/µg |
| Blue/white screening | No |
| Storage/Handling | Store Competent Cells at -80°C. |
AGL-1 (pSoup) Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-116-5x50 | 5 x 50 μL |
|
$ 156.00 |
| CC-116-10x50 | 10 x 50 μL |
|
$ 276.00 |
| CC-116-15x50 | 15 x 50 μL |
|
$ 370.00 |
Description
GoldBio’s AGL-1 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.
AGL-1 (pSoup) chemically competent cells are free of animal-derived products and grown with animal-free media.
The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the AGL-1 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: AGL-1 (pSoup)
Transformation efficiency: ≥3 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Product Specifications
| Catalog ID | CC-116 |
|---|---|
| Name(s) | AGL-1 (pSoup) Agrobacterium Chemically Competent Cells |
| Competent cell type | Chemically Competent |
| Species | A. tumefaciens |
| Transformation efficiency | ≥3 x 103 CFU/µg |
| Blue/white screening | No |
| Storage/Handling | Store Competent Cells at -80°C. |
