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AGL-1 Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-208-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-208-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-208-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted in the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥5 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-225-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-225-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-225-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s EHA105 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-207-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-207-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-207-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pmp90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted from the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101
Transformation efficiency: ≥2 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
LBA4404 Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-220-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-220-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-220-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s LBA4404 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: LBA4404
Transformation efficiency: ≥4 x 106 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Agrobacterium Electrocompetent Cells from GoldBio
GoldBio's Agrobacterium electrocompetent cells have ultra high efficiency, ensuring your Agrobacterium mediated plant transformations will run smoothly and effectively. Furthermore, we carry a variety of genomic competent cell strains so you can find the exact competent cells you need.
Currently available plant competent cell strains include ALG-1, EHA105, GV3101 and LBA4404. We also provide an Agrobacterium electrocompetent cell combination pack allowing you to test various Agrobacterium strains for your experiment.
Agrobacterium Competent Cell Genomic Strain Specifications
| Strains | Chromosomal Background | Ti Plasmid | Resistance | Plants |
| AGL-1 | C58 | pTiBO542 | Rifampicin, Carbenicillin |
Arabidopsis thaliana, maize, and other monocots |
| EHA105 | C58 | PpEHA105 (pTi(Bo542DT-DNA) |
Rifampicin | rice, tobacco, and other plants |
| GV3101 | C58 | pMP90 (pTiC58DT-DNA) |
Rifampicin, Gentamicin |
Arabidopsis thaliana, tobacco, potato, soybeans, and other plants |
| LBA4404 | Ach5 | pAL4404 | Rifampicin | tomatoes, tobacco, and other plants |
Additional Details
Storage/Handling: This product may be shipped on dry ice. Agrobacterium Electrocompetent cells should be stored at -80°C, pCAMBIA1391z Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.
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