|CC-208-5x50||5 x 50 μL||$ 199.00|
|CC-208-10x50||10 x 50 μL||$ 359.00|
|CC-208-15x50||15 x 50 μL||$ 485.00|
GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted in the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Transformation efficiency: ≥5 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells provide the highest transformation efficiencies ideal for applications such as cDNA or gDNA library construction.
The AGL-1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicillin resistance in its genome for selection. AGL-1 contains the Ti plasmid pTiBo542 from which the T-DNA region sequences have been deleted.
Transformation Efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
|Transformation Efficiency||≥1 x 107 cfu/µg pCAMBIA1391z DNA|
|Reagents for One Reaction||* AGL-1 Electrocompetent
Agrobacterium: 25 µl
* DNA (pCAMBIA1391z, 100 pg/µl): 1 µl
* Recovery medium: 1 ml
|Plasmid Information||Ti plasmid pTiBo542)|
|Resistance Information||rifampicin and carbenicillin resistance|
|Plant Information||Arabidopsis thaliana, maize,
and other monocots
Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.
Storage/Handling: This product may be shipped on dry ice. AGL-1 Agrobacterium Electrocompetent cells should be stored at -80°C, pCAMBIA1391z Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.